Abcam, ab308218, Anti-PIP5K1A antibody [HL2011]

Size: 100µL
Rabbit Recombinant Monoclonal PIP5K1 alpha/PIP5K1A antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Human, Mouse, Rat, Dog samples. Immunogen corresponding to Recombinant Fragment Protein within Human PIP5K1A.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:HL2011,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human, Dog,
Applications:WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human PIP5K1A. The exact immunogen used to generate this antibody is proprietary information.Q99755

Product details:
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PIP5K1 alpha also known as Phosphatidylinositol-4-phosphate 5-kinase type 1 alpha (PIP5K1A) is an enzyme that catalyzes the phosphorylation of phosphatidylinositol-4-phosphate (PI4P) to generate phosphatidylinositol-45-bisphosphate (PI(45)P2). The protein has an approximate mass of 68 kDa and is highly expressed in a variety of human tissues including the brain heart and spleen. PIP5K1A plays a significant role in lipid signaling and membrane dynamics impacting a wide array of cellular processes such as cytoskeletal organization and vesicle trafficking.
Biological function summary
PIP5K1A influences the production of PI(45)P2 an important phosphoinositide involved in numerous cellular functions. PI(45)P2 acts as a substrate for the production of secondary messengers like inositol trisphosphate (IP3) and diacylglycerol (DAG) essential in signal transduction pathways. PIP5K1A is part of a larger protein complex interacting with cytoskeletal elements and regulators of endocytosis and exocytosis. This association highlights its importance in maintaining cellular compartmentalization and function.
Pathways
PIP5K1A plays a pivotal role in the phosphoinositide signaling pathway and the actin cytoskeleton regulation pathway. It works closely with proteins such as RAC1 a small GTPase involved in actin polymerization to facilitate changes in the cell structure and motility. Additionally PIP5K1A's product PI(45)P2 serves as a precursor for multiple signaling molecules integrating with pathways that mediate cellular responses to various extracellular stimuli.
PIP5K1A's dysregulation links to cancer and neurological disorders. In cancer alterations in PIP5K1A expression or activity can affect cell proliferation and survival interacting with proteins like AKT that drive oncogenic signaling. In the context of neurological disorders its role in synaptic function and plasticity directly connects to conditions like schizophrenia where PI(45)P2 imbalance may contribute to the disorder's pathophysiology. These associations highlight the essential impact of PIP5K1A in maintaining cellular homeostasis and its potential as a therapeutic target.