Abcam, ab309115, Mouse TREM2 ELISA Kit - Extracellular

Size: 1 x 96Tests / 1 x 384Tests
Mouse TREM2 ELISA Kit - Extracellular is a single-wash 90-min Simplestep used to quantify Mouse TREM2 with a sensitivity of 3.2 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Different formats for different needs: 384-well plate for higher throughput - Validated on a number of sample types including cerebrospinal fluid (CSF) - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Cerebral Spinal Fluid, Cell culture supernatant,
Reacts with:Mouse,
Assay type:Sandwich (quantitative),
Sensitivity:= 3.2 pg/mL,
Range:12.5 - 800 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Mouse TREM2 ELISA Kit - Extracellular ab309115 is a rapid single-wash 90-min Sandwich ELISA to measure Mouse TREM2 - Extracellularin cell culture supernatant, CSF. This SimpleStep sensitivity is 3.2 pg/mL.
How the assay works
Mouse TREM2 - ExtracellularSimpleStep ELISA
employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA
plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA
protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA
kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Mouse TREM2 ELISA Kit - Extracellular ab309115 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
Save your precious samples
Our 384-well Mouse TREM2 ELISA Kit - Extracellular only requires a maximum of 12.5µL sample

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C