Abcam, ab309309, Human Fibrinogen gamma chain ELISA Kit - Intracellular

Size: 1 x 96Tests
Human Fibrinogen gamma chain ELISA Kit - Intracellular is a single-wash 90-min Simplestep used to quantify Human Fibrinogen gamma chain with a sensitivity of 8.8 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Cell Lysate, Tissue Extracts,
Reacts with:Human,
Assay type:Cell-based (quantitative),
Sensitivity:= 8.8 pg/mL,
Range:43.75 - 2800 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human Fibrinogen gamma chain - Intracellular SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Fibrinogen gamma chain - Intracellular protein in human serum, plasma, saliva and cell culture supernatant. Quantitate Human Fibrinogen gamma chain - Intracellular with 8.8 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (
ab203359
) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
REACTIVITY
2,300 pg/mL of recombinant Human Fibrinogen alpha was tested for cross reactivity. No cross reactivity was observed.
2,300 pg/mL of recombinant Human Fibrinogen beta was tested for cross reactivity. <3% cross reactivity was observed.
INTERFERENCE
575 pg/mL of recombinant Human Fibrinogen alpha and 575 pg/mL of recombinant Human Fibrinogen beta was tested for interference with 575 pg/mL of recombinant Human Fibrinogen gamma. No interference was observed. 575 pg/mL of recombinant Human Fibrinogen beta increased interpolated value of 575 pg/mL of recombinant Human Fibrinogen gamma 5.7%.
SPECIES REACTIVITY
Other species reactivity was determined by measuring 1:1000 serum samples of various species, interpolating the protein concentrations from the species standard curve, and expressing the interpolated concentrations as a percentage of the protein concentration in species serum assayed at the same dilution.
Reactivity of 77% was determined for primate serum.
No signal was observed in 1:1000 serum samples from the following species: Mouse, Rat, Bovine
Other species reactivity not determined.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C