Abcam, ab309532, Anti-Deptor antibody [EPR26159-220] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal Deptor antibody. Carrier free. Suitable for WB, IHC-P, IP and reacts with Human, Rat samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR26159-220,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human, Rat,
Applications:IP, WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4 Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
DEPTOR also known as DEP domain-containing mTOR-interacting protein functions as a subunit of the mTOR (mechanistic Target of Rapamycin) complexes. This protein has a molecular weight of approximately 48 kDa and is expressed extensively in tissues such as skeletal muscle adipose tissue and the brain. DEPTOR directly interacts with the mTOR complexes modulating their activity. Its exact mechanical role involves binding to mTOR and influencing the assembly and function of mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2).
Biological function summary
DEPTOR is important for the regulation of cell growth and metabolism. It is a component of the mTORC1 and mTORC2 two key protein complexes that control cell proliferation survival and autophagy. DEPTOR acts as an inhibitor within these complexes reducing their activity. By regulating the mTOR signaling pathway it helps balance anabolic and catabolic processes within the cell. Without proper DEPTOR function cells may grow unchecked leading to various issues including metabolic dysregulation.
Pathways
DEPTOR plays a significant role in the mTOR signaling pathway and the PI3K/AKT pathway. In the mTOR pathway DEPTOR controls the activity of mTORC1 and mTORC2 which are essential for translating signaling into cellular response. The PI3K/AKT pathway also involves DEPTOR as it relates to insulin signaling and glucose homeostasis. Proteins such as AKT and S6K work in this complex to transmit signals impacting numerous cellular activities. The dynamic balance it maintains within these pathways is critical for normal cellular function and metabolism.
DEPTOR is associated with conditions such as cancer and type 2 diabetes. In cancers its reduced expression allows for unchecked mTOR activity driving tumor growth and survival. Conversely unregulated expression can lead to increased cell apoptosis. DEPTOR's dysregulation in the context of type 2 diabetes affects glucose metabolism through interactions with the PI3K/AKT pathway. It also interacts with proteins like IRS-1 influencing insulin sensitivity and leading to metabolic disorders. DEPTOR's regulation of these pathways suggests potential therapeutic targets in these diseases.