Abcam, ab31217, Anti-eIF4A1 antibody

Size: 100µg
Anti-eIF4A1 antibody (ab31217) is a rabbit polyclonal antibody detecting eIF4A1 in Western Blot, IP, IHC-P, ICC/IF . Suitable for African green monkey, Cow, Human, Mouse, Rat . - Over 50 publications - Trusted since 2006
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human, African green monkey, Cow,
Applications:IHC-P, IP, ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:Replenishment batches of our polyclonal antibody, ab31217 are tested in WB. Previous batches were additionally validated in ICC/IF, IHC-P and IP. These applications are still expected to work and are covered by our Abpromise guarantee. You may also be interested in our alternative recombinant antibody, ab185946.

Product details:
What is this antibody validated in?
Anti-eIF4A1 antibody (ab31217) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in African green monkey, Cow, Human, Mouse, Rat samples.
What is the molecular weight of eIF4A1?
Anti-eIF4A1 (ab31217) specifically detects a band for eIF4A1 (UniProt: P60842) at a molecular weight of 47kDa.
Trusted by the scientific community
Anti-eIF4A1 (ab31217) was first used in a scientific publication in 2006 and has been cited over 50 times in peer-reviewed journals.
Reviewed by scientists
Anti-eIF4A1 (ab31217) has over 5 independent reviews from customers.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The eukaryotic initiation factor 4A1 also known as eIF4A1 is an essential RNA helicase part of the DEAD-box family. It unwinds secondary structures in the 5' untranslated region of mRNA facilitating ribosome binding during translation initiation. This protein has a molecular mass of approximately 46 kDa. Expressed ubiquitously in eukaryotic cells eIF4A1 plays a central role in regulating protein synthesis by enhancing the accessibility of mRNA templates to the ribosome.
Biological function summary
The eIF4A1 protein functions as a component of the eIF4F complex which includes eIF4E and eIF4G. This complex modulates the initiation of mRNA translation a critical step in gene expression. By associating with other initiation factors eIF4A1 helps orchestrate the recruitment of the ribosome to capped mRNA. Its activity influences cell growth proliferation and response to environmental signals.
Pathways
EIF4A1 involves in the regulation of the mTOR signaling pathway which controls cell growth based on nutrient availability. It also participates in the integrated stress response pathway linking stress signals to translational control. eIF4A1 interacts with other proteins such as eIF4E and eIF4G through its function within these pathways highlighting its role in translation regulation.
EIF4A1 has been implicated in cancer and neurodegenerative diseases. Aberrant regulation of eIF4A1 expression and activity contributes to oncogenesis by promoting uncontrolled protein synthesis and cell proliferation. In neurodegenerative disorders altered eIF4A1 activity disrupts normal neuronal function and viability. Connections to proteins like eIF4E highlight its impact on disease progression emphasizing the importance of understanding eIF4A1's functional mechanisms in these contexts.