Abcam, ab312857, Anti-Influenza A Virus PB2 protein antibody [EPR28249-46] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal PB2 antibody. Carrier free. Suitable for WB, IHC-P, Flow Cyt (Intra), ICC/IF, I-ELISA and reacts with Influenza A, Transfected cell lysate - Influenza A, Transfected cell line - Influenza A, Recombinant fragment - Influenza A samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR28249-46,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Influenza A,
Applications:ICC/IF, WB, Flow Cyt (Intra), IHC-P, I-ELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Influenza A Polymerase basic protein 2 also known as PB2 protein plays an important mechanical role in the viral RNA polymerase complex which is necessary for viral replication. This protein has a molecular mass of approximately 87 kDa. PB2 is expressed within infected host cells and is a component of the influenza A virus polymerase complex along with PA and PB1 proteins. The PB2 target is essential for the transcription and replication phase in the viral lifecycle allowing the virus to hijack the host cell machinery for viral propagation.
Biological function summary
PB2 protein is part of the heterotrimeric RNA polymerase complex which is critical for the influenza virus's ability to replicate. The polymerase complex includes PA PB1 and PB2 proteins where PB2 protein plays a role in cap-snatching a mechanism to steal host mRNA caps for viral mRNA synthesis. This enables the influenza virus to synthesize its own proteins in the host cytoplasm facilitating efficient viral replication and protein expression.
Pathways
The PB2 protein functions prominently within the viral replication cycle of the influenza A virus. It is intricately linked with the host's mRNA processing pathways as it engages in the cap-snatching mechanism necessary for viral mRNA transcription. PB2 protein works closely with PB1 in the replication pathway where PB1 acts as the RNA-dependent RNA polymerase in conjunction with activities of PB2.
PB2 protein plays a significant role in the pathogenicity of influenza A virus infections in humans and animals. Its interactions are pivotal for the adaptation and virulence of the influenza virus leading to significant morbidity during outbreaks. Mutations in the PB2 gene can relate to species adaptation and increased virulence and the protein's function is important for the virus to overcome host defense mechanisms frequently. PB2 interacts with host proteins to moderate host immune responses making it a target for antiviral drug development and treatment strategies.