Abcam, ab312890, Alexa Fluor® 488 Anti-STAT5a + STAT5b antibody [EPR16671-40]

Size: 100µL
Rabbit Recombinant Monoclonal STAT5a antibody - conjugated to Alexa Fluor® 488. Suitable for Flow Cyt (Intra) and reacts with Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR16671-40,
Isotype:IgG,
Conjugation:Alexa Fluor® 488,
Excitation/Emission:Ex: 495nm, Em: 519nm,
Carrier free:No,
Reacts with:Human,
Applications:Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle, Store in the dark

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
STAT5a and STAT5b known jointly as members of the Signal Transducers and Activators of Transcription family play critical roles as transcription factors. They have molecular masses of approximately 94 kDa and 92 kDa respectively. These proteins share significant sequence similarity and are expressed in a wide range of tissues including hematopoietic cells liver and mammary glands. STAT5 proteins become activated in response to cytokines and growth factors where they dimerize and translocate to the cell nucleus to regulate gene expression.
Biological function summary
They are involved in mediating cytokine signaling by forming homo- or heterodimers upon phosphorylation by Janus kinases (JAKs). In the cytoplasm the formation of these dimers allows STAT5 to interact with DNA in the nucleus leading to the transcription of various genes important for cell proliferation differentiation and apoptosis. STAT5 has a significant role in cell cycle control and immune responses. The dimerization process involves complex formation and is an important part of its intracellular signaling cascade.
Pathways
STAT5a and STAT5b operate prominently within the JAK-STAT pathway. They are activated by proteins such as IL-2 IL-3 and Growth Hormone leading to transcriptional activity that affects growth and survival across various cell types. Besides the JAK-STAT pathway STAT5 interacts closely with the PI3K/AKT pathway thereby linking to cell survival signals. The cross-talk between these pathways enables fine-tuning of cellular responses to external stimuli and integrates signals from diverse receptors and ligands.
STAT5 deregulated activity associates with various cancers such as leukemia and breast cancer due to its role in promoting excessive cell proliferation and survival. Abnormal activity in STAT5 can also contribute to disorders like autoimmune diseases due to its impact on immune cell regulation. In diseases like these STAT5 interacts notably with proteins such as BCL-2 and MYC which are critical modulators of cell death and growth pathways. Understanding the involvement of STAT5 in disease mechanisms provides opportunities for developing targeted therapeutics.