Abcam, ab312893, Alexa Fluor® 488 Anti-AKT1 + AKT2 antibody [EPR18405]

Size: 100µL
Rabbit Recombinant Monoclonal AKT1 phospho S473 antibody - conjugated to Alexa Fluor® 488. Suitable for Flow Cyt (Intra) and reacts with Human, Mouse samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR18405,
Isotype:IgG,
Conjugation:Alexa Fluor® 488,
Excitation/Emission:Ex: 495nm, Em: 519nm,
Carrier free:No,
Reacts with:Human, Mouse,
Applications:Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle, Store in the dark

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The AKT1 and AKT2 proteins also known as Protein Kinase B (PKB) isoforms are serine/threonine kinases with molecular masses approximately 56 kDa each. These proteins play mechanical roles as critical regulatory components in cell signaling pathways. AKT1 and AKT2 get activated via phosphorylation events particularly at threonine 308 and serine 473 for AKT1 facilitating downstream effects. AKT1 and AKT2 are expressed ubiquitously across diverse tissue types including heart brain lung and other vital organs. Their cellular presence ensures robust signaling responses across different physiological contexts.
Biological function summary
AKT1 and AKT2 regulate key cellular processes such as glucose metabolism apoptosis and cell proliferation. The two proteins act as part of the complex involving phosphoinositide 3-kinase (PI3K) affecting the survival and growth of cells. Through their actions these kinases impact various cellular mechanisms maintaining homeostasis and cellular integrity under stress. Their ability to modulate such essential processes illustrates their significance in maintaining cellular life and function.
Pathways
AKT1 and AKT2 are integral components of the PI3K/AKT/mTOR signaling pathway a pathway relevant in the regulation of cell growth and survival. Within this pathway AKT1 and AKT2 interact closely with proteins such as PI3K and mTOR executing signaling cascades essential for normal cellular functions. Additionally they exhibit connections to the insulin signaling pathway where they interact with insulin receptor substrates (IRSs) influencing glucose uptake and energy balance within the cell.
AKT1 and AKT2 have substantial relevance to several pathological conditions such as cancer and type 2 diabetes. Aberrant expression or mutations in AKT1 and AKT2 can lead to uncontrolled cell proliferation contributing to oncogenesis. Likewise these proteins affect insulin signaling and dysregulation can result in insulin resistance and type 2 diabetes. During these disease states AKT1 and AKT2 show considerable interactions with proteins like PTEN a phosphatase that negatively regulates the PI3K/AKT pathway highlighting their roles in pathophysiological conditions.