Abcam, ab313373, Anti-RG9MTD1 antibody [EPR27065-61] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal TM10C antibody. Carrier free. Suitable for IHC-P, Flow Cyt (Intra), WB, ICC/IF, IP and reacts with Human, Mouse, Rat samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR27065-61,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human, Mouse, Rat,
Applications:ICC/IF, IP, WB, IHC-P, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:Unsuitable for mouse and rat IHC-P.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
RG9MTD1 also known as G9a or EHMT2 is an enzyme with a molecular mass of approximately 150 kDa. It is a histone methyltransferase that specifically targets lysine residues on histone H3. This methylation leads to chromatin modification and affects gene expression. RG9MTD1 is predominantly expressed in the nucleus of cells and is found in a variety of tissues across the body including the brain liver and kidney.
Biological function summary
The enzyme RG9MTD1 contributes to the regulation of transcription by modifying chromatin structure. It is a component of the larger transcriptional repressor complex that includes other proteins like GLP (G9a-like protein). This complex mediates the dimethylation of histone H3 lysine 9 (H3K9me2) which plays a role in gene silencing. The presence of RG9MTD1 in this complex indicates its importance in controlling various genetic programs within different cell types.
Pathways
The activity of RG9MTD1 is important in key signaling pathways including the transforming growth factor-beta (TGF-beta) pathway and Wnt signaling. In the TGF-beta pathway RG9MTD1 interacts with proteins such as Smad to influence transcriptional responses impacting cell growth and differentiation. Similarly in the Wnt signaling pathway RG9MTD1 affects the expression of target genes that are essential for cell proliferation and embryonic development.
The deregulation of RG9MTD1 is connected to various cancers such as lung and colorectal cancer. Abnormal methylation patterns influenced by RG9MTD1 activity can lead to the silencing of tumor suppressor genes. Additionally neurodevelopmental disorders including autism spectrum disorders show links with RG9MTD1 due to its role in brain development and gene regulation. The interaction between RG9MTD1 and other proteins like SUV39H1 is significant in understanding its impact on disease pathogenesis.