Abcam, ab313575, Anti-RAB27A + RAB27B antibody [EPR27120-68]

Size: 20µL / 100µL / 1mL
Rabbit Recombinant Monoclonal RAB27A antibody. Suitable for IHC-P, WB, IP, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat, Transfected cell lysate - Human, Transfected cell line - Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR27120-68,
Isotype:IgG,
Carrier free:No,
Reacts with:Human, Mouse, Rat,
Applications:Flow Cyt (Intra), ICC/IF, IP, WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
RAB27A and RAB27B are members of the Rab protein family which are small GTPases involved in membrane trafficking. These proteins also known as GTP-binding proteins are critical for vesicle transport and membrane fusion processes. RAB27A has a molecular mass of approximately 25 kDa and RAB27B has a molecular mass around 24.5 kDa. Both proteins express widely in various cell types yet show higher expression in secretory cells particularly in the brain pancreas and melanocytes. RAB27A and RAB27B localize to the membrane of secretory granules aiding in their mobilization.
Biological function summary
The proteins RAB27A and RAB27B facilitate the movement of secretory vesicles toward the cell membrane for exocytosis. RAB27A is a component of protein complexes known as exocyst complexes which are involved in tethering vesicles to the plasma membrane. The proteins recruit effector molecules such as Myosin Va and Slp2-a to the vesicle surface enhancing their docking and fusion with the cell membrane. This process is essential for regulated secretion of hormones neurotransmitters and other important molecules.
Pathways
RAB27A and RAB27B play important roles in the vesicular transport systems that govern secretion pathways including the exocytosis and endocytosis pathways. During exocytosis RAB27A works closely with SNARE proteins to stabilize vesicle fusion. It also interacts with other Rab proteins like RAB3D contributing to efficient vesicle trafficking. In addition RAB27B has a role in synaptic vesicle pathways which are key to neurotransmitter release in neural communication.
Mutations in RAB27A lead to Griscelli syndrome type 2 characterized by immune system dysfunction and pigment dilution. This syndrome occurs due to defective vesicle transport in melanocytes and immune cells. RAB27A is linked with LYST a protein involved in lysosomal trafficking which causes Chediak-Higashi syndrome when mutated. Moreover altered RAB27B expression associates with colorectal cancer progression emphasizing its importance in cellular secretion dysfunctions.