Abcam, ab313622, Anti-PUS10 antibody [EPR26272-66]

Size: 20µL / 100µL / 1mL
Rabbit Recombinant Monoclonal PUS10 antibody. Suitable for WB, IP, Flow Cyt (Intra), IHC-P and reacts with Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR26272-66,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IP, Flow Cyt (Intra), IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The protein PUS10 also known as pseudouridylate synthase 10 catalyzes the isomerization of uridine to pseudouridine in RNA molecules. This process introduces pseudouridine modifications critical for RNA stability and processing. PUS10 has a molecular mass of approximately 57 kDa and it is expressed in several tissues including the heart and skeletal muscle. This protein is found in both the nucleus and cytoplasm of cells suggesting its role in modifying different types of RNA.
Biological function summary
PUS10 plays significant functions that affect RNA biogenesis and stability. Its involvement in pseudouridylation impacts the structure and function of tRNA rRNA and other small RNA species. This protein is not part of a larger protein complex but works independently. Its activity influences the integrity of RNA molecules which is important for accurate protein synthesis and cellular function.
Pathways
PUS10 significantly contributes to RNA modification and processing pathways. It is a part of the wider RNA maturation process where it cooperates with other RNA-modifying enzymes like dyskerin (DKC1). DKC1 also participates in pseudouridylation highlighting the interconnected roles in ensuring RNA’s proper folding and functionality. PUS10’s involvement in the RNA modification pathway underlines its importance in maintaining RNA function integrity.
PUS10 has been linked to several health conditions. It has notable associations with cancer and autoimmune diseases. In cancer alterations in PUS10 expression levels can disrupt normal cell function potentially leading to tumorigenesis. It also interacts with the p53 protein which plays a role in tumor suppression. In autoimmune diseases the misregulation of RNA modifications including pseudouridylation by PUS10 might contribute to autoimmune responses due to altered RNA structures. Understanding PUS10 in these contexts provides insight into how RNA modifications could influence disease progression and treatment.