Abcam, ab313676, Human CRHBP ELISA Kit

Size: 1 x 96Tests
Human CRHBP ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human CRHBP in Human in Cell Culture Media, Biofluids samples.
Key facts
Detection method:Colorimetric,
Sample types:Cell culture supernatant, Plasma, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 0.07 ng/mL,
Range:0.07 - 20 ng/mL,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human CRHBP ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CRHBP in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human CRHBP coated on a 96-well plate.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CRHBP also known as Corticotropin-Releasing Hormone Binding Protein plays a role in regulating the activity of corticotropin-releasing hormone (CRH) by binding to it with high affinity. The binding protein has a molecular mass of approximately 37 kDa. It is mainly found in the brain and pituitary gland. CRHBP controls the actions of CRH by sequestering it preventing CRH from interacting with its receptors. This modulation is essential for maintaining the balance of CRH's activity.
Biological function summary
CRHBP modulates the stress response by binding to CRH and limiting its availability to CRHR1 and CRHR2 receptors. By doing so it reduces the downstream signaling that would be induced by free CRH which affects the release of adrenocorticotropic hormone (ACTH) from the pituitary. This regulation is important for controlling how the body responds to stress. CRHBP does not form a larger protein complex but operates by interacting directly with CRH in the extracellular space.
Pathways
CRHBP functions within the hypothalamic-pituitary-adrenal (HPA) axis which plays an important role in the body's response to stress. By regulating CRH availability CRHBP indirectly influences the cortisol production pathway. This is important because cortisol is an important hormone in modulating metabolism and immune responses. Furthermore CRHBP interactions connect it to CRH which also communicates with other stress-related proteins such as CRHR1 and CRHR2 influencing their activity within the HPA axis.
CRHBP has been linked to conditions such as depression and anxiety disorders. The protein's role in regulating stress responses makes it relevant for understanding these conditions. Changes in CRHBP expression or function may alter CRH availability impacting mood regulation and psychological resilience. Additionally CRH the ligand for CRHBP is implicated in these disorders by influencing pathways that affect emotional behavior and stress tolerance.