Abcam, ab313754, Alexa Fluor® 488 Anti-IgG2 antibody [EPR4418]

Size: 100µL
Rabbit Recombinant Monoclonal IGHG2 antibody - conjugated to Alexa Fluor® 488. Suitable for IHC-P, Flow Cyt (Intra) and reacts with Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR4418,
Isotype:IgG,
Conjugation:Alexa Fluor® 488,
Excitation/Emission:Ex: 495nm, Em: 519nm,
Carrier free:No,
Reacts with:Human,
Applications:Flow Cyt (Intra), IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle, Store in the dark

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
IgG2 also known as Immunoglobulin G2 is a subclass of the IgG antibodies within the immune system. It is a glycoprotein with a molecular mass typically around 150 kDa. As it is part of the IgG class IgG2 contributes to the body's defense mechanisms by binding to specific antigens. The expression of IgG2 occurs in plasma cells derived from B cells following antigen exposure. It circulates in the blood and extracellular fluid playing an important role in identifying and neutralizing pathogens such as bacteria and viruses.
Biological function summary
IgG2 plays an essential role in the immune response by activating the complement system therefore facilitating opsonization and eventual phagocytosis of pathogens. IgG2 forms part of the immunoglobulin family complex which mediates immune responses. It exhibits lower affinity for Fc receptors on phagocytic cells compared to other IgG subclasses. This characteristic affects its ability to mediate antibody-dependent cellular cytotoxicity. Despite this IgG2 remains key in recognizing polysaccharide antigens typically found on bacterial capsules which enhances immune response specificity.
Pathways
IgG2 acts within the adaptive immune system involving humoral immunity pathways. It participates in the classical complement activation pathway leading to the destruction of antigens through membrane attack complexes. IgG2 interacts with other immunoglobulin family members including IgG1 and IgG3 through these pathways offering a comprehensive immune response. The relationship with IgM is also notable as both IgG2 and IgM are involved in the first line of defense against polysaccharide antigens.
IgG2 levels and function relate to conditions such as recurrent bacterial infections and IgG subclass deficiencies. Patients with specific IgG2 deficiencies often suffer from increased susceptibility to infections particularly those caused by encapsulated bacteria like Streptococcus pneumoniae. Autoimmune diseases such as Systemic Lupus Erythematosus (SLE) may also involve dysfunctional IgG2 responses linking IgG2 with proteins like C1q in disease pathology. Researchers continue to study these connections to better understand immune function and improve therapeutic strategies.