Abcam, ab313877, Anti-SA2 (isoform-2) antibody [EPR26892-61]

Size: 20µL / 100µL / 1mL
Rabbit Recombinant Monoclonal SA2 antibody. Suitable for ChIP-seq, Flow Cyt (Intra), IHC-P, WB, IP, ICC/IF and reacts with Human, Mouse, Rat, Transfected cell line - Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR26892-61,
Isotype:IgG,
Carrier free:No,
Reacts with:Human, Mouse, Rat,
Applications:Flow Cyt (Intra), WB, IP, ICC/IF, ChIP-seq, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:Unsuitable for human IHC-P.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SA2 isoform-2 also known as Structural Maintenance of Chromosomes Protein 3 (SMC3) is a component of the cohesin complex which plays a critical role in the cohesion of sister chromatids. SA2 isoform-2 has a mass of approximately 150 kDa. It is expressed in a variety of tissues with significant expression in dividing cells where its function contributes to proper cell division and chromosome segregation. The capacity of SA2 isoform-2 to bind and stabilize cohesion complexes in the nucleus is fundamental for maintaining genomics stability during the cell cycle.
Biological function summary
SA2 isoform-2 supports the integrity of chromatid pairing. As part of the cohesin complex it interacts with other cohesin subunits such as SMC1 SMC3 and RAD21. This complex regulates chromosome architecture by facilitating loop formation and sister chromatid cohesion. These functionalities are essential during the cell cycle particularly in the S and G2 phases ensuring that chromatid pairs do not prematurely separate before mitosis.
Pathways
SA2 isoform-2 plays an important role in cell cycle regulation and DNA damage repair pathways. Within the cell cycle its function is connected to the loading and stabilization of cohesin complexes that enable proper chromosome alignment and segregation during mitosis. In DNA repair pathways it associates with proteins like PDS5 and WAPL which contribute to modulating cohesin dynamics and ensuring successful repair of DNA double-strand breaks. These activities are pivotal to preserving chromosomal stability.
Mutations or dysfunctions in SA2 isoform-2 are linked to cohesinopathies including Cornelia de Lange Syndrome and certain cancer types. In Cornelia de Lange Syndrome mutations affecting SA2 isoform-2 or its interacting partners such as NIPBL result in developmental abnormalities and intellectual disability. In cancer aberrant expression or malfunctions in components of the cohesin complex which includes SA2 isoform-2 can lead to genomic instability promoting tumorigenesis and driving cancer progression.