Abcam, ab313879, Anti-XAF1 antibody [EPR26960-175]

Size: 20µL / 100µL / 1mL
Rabbit Recombinant Monoclonal XAF1 antibody. Suitable for WB, IP and reacts with Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR26960-175,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The XAF1 protein also known as XIAP associated factor 1 plays a role in apoptosis regulation. It weighs approximately 34 kDa and exists in multiple isoforms. The protein is broadly expressed in various tissues including the liver pancreas and heart. XAF1 interacts directly with XIAP (X-linked inhibitor of apoptosis) counteracting its anti-apoptotic effects. XAF1's activity centers on promoting apoptosis by binding to XIAP and inhibiting its function.
Biological function summary
XAF1 functions in promoting cell death through apoptosis. It forms part of the apoptosome a large protein complex important for the intrinsic pathway of apoptosis. XAF1 exerts its influence by destabilizing XIAP's interaction with caspases releasing them to activate. These interactions trigger a cascade that leads to programmed cell death vital for maintaining cellular balance and response to stress.
Pathways
XAF1 plays significant role in the apoptosis and cell survival pathways. Within the intrinsic pathway XAF1 associates with Bax and caspase-3 directly impacting mitochondrial membrane permeability and caspase activation. These pathways ensure proper cell turnover and reaction to cellular damage often involving Bcl-2 family proteins and Smac/DIABLO protein for regulation as well.
XAF1's role in cancer and neurological disorders is noteworthy. In several cancer types such as colon and gastric cancers decreased XAF1 expression associates with increased cancer cell survival due to impaired apoptosis. Meanwhile abnormal regulation of XAF1 links to neurodegenerative diseases involving proteins like XIAP and caspase-9 where inhibited apoptosis can lead to accumulation of damaged cells contributing to disease progression.