Abcam, ab313967, Human Glypican 5 ELISA Kit

Size: 1 x 96Tests
Human Glypican 5 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human Glypican 5 in Human in Biofluids, Cell Culture Media samples.
Key facts
Detection method:Colorimetric,
Sample types:Serum, Plasma, Cell culture supernatant,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 1.3 ng/mL,
Range:1.3 - 300 ng/mL,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human Glypican 5 ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Glypican 5 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human Glypican 5 coated on a 96-well plate.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Glypican 5 (GPC5) is a cell surface heparan sulfate proteoglycan that is part of the glypican family. This protein weighs about 125 kDa. It is found mainly in the central nervous system and lung tissues. Glypicans including GPC5 are bound to the cell membrane via a glycosylphosphatidylinositol (GPI) anchor playing roles in cellular signaling at the cell surface.
Biological function summary
Glypican 5 modulates cell growth and differentiation by interacting with growth factors and morphogens acting as a coreceptor. It collaborates with heparan sulfate chains enabling the binding of signaling molecules at the cell surface. GPC5 does not work alone; it forms complexes with extracellular matrix components to influence cellular behavior. Its role extends to effect changes in the cellular microenvironment impacting development and tissue homeostasis.
Pathways
Glypican 5 serves as a part of two important pathways: the Wnt and Hedgehog signaling pathways. These pathways are critical for embryonic development and tissue patterning. GPC5 interacts with proteins like Frizzled and Patched which are receptors within these pathways. The interaction enhances signaling efficiency and contributes to the precise regulation of developmental processes.
Glypican 5 has links to cancers and congenital malformations. Abnormal expression of GPC5 has associations with lung cancer where it affects tumor growth and metastasis. Additionally GPC5 mutations or altered expression can lead to developmental disorders such as Simpson-Golabi-Behmel syndrome. In these conditions GPC5 interacts with proteins like Sonic Hedgehog complicating the disease mechanisms and influencing treatment strategies.