Abcam, ab314125, Anti-DOM3Z antibody [EPR27511-140] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal DOM3Z antibody. Carrier free. Suitable for IHC-P, ICC/IF, Flow Cyt (Intra), WB and reacts with Mouse, Rat, Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR27511-140,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:Flow Cyt (Intra), WB, IHC-P, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:Unsuitable for human IHC-P, mouse ICC and mouse FC-intra

Product details:
ab314125 is the carrier-free version of
ab314124
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
DOM3Z also known as exosome component 1 or hDOM3Z is a protein involved in RNA processing and degradation. It has a molecular mass of about 28 kDa. This protein is present in various tissues with higher expression levels seen in the heart and skeletal muscle. DOM3Z localizes to the nucleus where it plays an important role in maintaining RNA quality control. The protein acts within the RNA exosome complex which is a multi-protein complex essential for RNA decay.
Biological function summary
DOM3Z forms part of the RNA exosome complex working in concert with other components to remove defective or unnecessary RNA molecules. This activity is essential for regulating RNA homeostasis and ensuring the fidelity of RNA populations within the cell. By interacting with exosome core components and exosome co-factors DOM3Z facilitates the degradation of a wide range of RNA substrates including messenger RNA and non-coding RNA. The proper function of DOM3Z within the exosome complex assures accuracy in gene expression regulation.
Pathways
The RNA degradation pathway heavily relies on functions executed by DOM3Z. It interacts with related proteins such as RRP6 and RRP44 within the exosome assembly. Another significant pathway involving DOM3Z is the non-coding RNA processing pathway where its involvement ensures the correct biogenesis and maturation of small nuclear RNAs and small nucleolar RNAs. This involvement of DOM3Z and its associated proteins highlights its central role in maintaining cellular RNA integrity and gene expression equilibrium.
Abnormalities in DOM3Z function correlate with neuromuscular disorders reflecting its higher expression levels in muscle tissues. Mutations or misregulation that affect DOM3Z can also relate to certain types of cancer where disrupted RNA processing leads to uncontrolled cell proliferation. In the context of these conditions DOM3Z interacts with several proteins like DIS3L2 which play roles in disease mechanisms. Understanding and investigating these associations may offer new avenues for therapeutic strategies targeting RNA metabolism disorders.