Abcam, ab314316, Anti-ENPP2/ATX antibody [EPR27348-30] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal ENPP2/ATX antibody. Carrier free. Suitable for Flow Cyt (Intra), ICC/IF, WB and reacts with Human, Mouse samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR27348-30,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human, Mouse,
Applications:ICC/IF, Flow Cyt (Intra), WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The ENPP2 protein also known as autotaxin (ATX) with a mass of approximately 102 kDa mainly manifests as a secreted enzyme with lysophospholipase D activity. ENPP2 or ATX-001 widely expresses in various tissues predominantly in the brain adipose tissue and placenta. It catalyzes the hydrolysis of lysophosphatidylcholine to lysophosphatidic acid (LPA) and choline playing an important role in LPA signaling. ENPP2/ATX-001 is a valuable component in the study of lipid signaling pathways.
Biological function summary
ATX protein influences cell proliferation migration and survival. It does not operate as part of a complex but directly impacts the biological processes previously mentioned due to its enzymatic function. It acts significantly during embryonic development and wound healing. The products of its catalysis LPA engage in various signaling pathways essential for these processes.
Pathways
ENPP2 integrates into the lysophospholipid and phospholipase pathways. It plays an important role in the LPA receptor signaling pathway contributing to various physiological and pathological processes. Proteins related to ENPP2 through these pathways include LPA receptors such as LPAR1 and LPAR2 with which it interacts closely to mediate cell signaling roles and phospholipase A2 which provides substrate for its enzymatic activity.
ENPP2 links strongly with cancer and fibrotic diseases. Its enzymatic activity generates LPA which can enhance tumor progression by promoting cell proliferation and migration. This activity associates ENPP2 with oncogenic processes. Additionally LPA signaling stands connected to fibrotic disorders where it contributes to tissue remodeling and fibrosis. Its overactivity or dysregulation might result in such pathological states associating indirectly with growth factor proteins like TGFB1 which are involved in tissue fibrosis and cancer.