Abcam, ab314368, Human Granzyme K ELISA Kit

Size: 1 x 96Tests
Human Granzyme K ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Granzyme K with a sensitivity of 6.1 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Serum, Citrate plasma, Heparin Plasma, EDTA Plasma, Cell culture supernatant,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 6.1 pg/mL,
Range:34.375 - 2200 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human Granzyme K ELISA Kit ab314368 is a rapid single-wash 90-min Sandwich ELISA to measure Human Granzyme K in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 6.1 pg/mL.
How the assay works
Human Granzyme K SimpleStep ELISA
employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA
plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA
protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA
kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Human Granzyme K ELISA Kit ab314368 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Granzyme K also known as protein K or 24c3 is a serine protease with a mass of approximately 27 kilodaltons. It belongs to the granzyme family of proteins which are mainly found within the cytotoxic granules of natural killer (NK) cells and cytotoxic T lymphocytes (CTLs). These immune cells store granzyme K in their granules which they release to induce apoptosis in target cells. Researchers have identified granzyme K expression in various tissues and immune cells highlighting its widespread role in modulating immune responses.
Biological function summary
Granzyme K serves as a potent mediator in the immune system by inducing apoptosis in target cells. It does not act alone but rather in concert with other granzymes and proteins to effectively execute cell death. Granzyme K can degrade cellular proteins once introduced into a target cell's cytoplasm leading to apoptosis. The protease functions independently and does not require a complex for its activity contributing to the immune system's ability to eliminate infected or transformed cells.
Pathways
The granzyme K protein exists within essential immune regulatory pathways such as the cytotoxic T lymphocyte-induced apoptosis pathway and the natural killer cell-mediated cytotoxicity pathway. Granzyme K acts alongside granzymes A and B to promote programmed cell death and finely tune host immune responses. While granzymes A and B have more defined roles granzyme K complements their activity offering redundancy and ensuring efficient elimination of harmful target cells.
Granzyme K shows significant connections to inflammatory conditions and autoimmune diseases such as rheumatoid arthritis. Elevated granzyme K levels in these diseases may result from dysregulated immune responses leading to excessive tissue damage. In autoimmune disorders granzyme K works in combination with proteins like perforin which facilitates entry of granzymes into the cell to stimulate apoptosis. Understanding granzyme K's role in these conditions may help develop therapeutic approaches to modulate immune activity and minimize tissue damage.