Abcam, ab314402, PE Anti-AKT1 + AKT2 antibody [EPR18405]

Size: 100µL
Rabbit Recombinant Monoclonal AKT2 antibody - conjugated to PE. Suitable for Flow Cyt (Intra) and reacts with Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR18405,
Isotype:IgG,
Conjugation:PE,
Excitation/Emission:Ex: 480;565nm, Em: 578nm,
Carrier free:No,
Reacts with:Human,
Applications:Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle, Store in the dark

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
AKT1 and AKT2 also known as Protein Kinase B (PKB) family members are serine/threonine-specific kinases of about 56 kDa. They are expressed in many tissues with higher levels in muscle brain and heart. Mechanically AKT1 and AKT2 function in cellular signaling by being activated through phosphoinositide 3-kinase (PI3K)-dependent processes leading to phosphorylation of downstream substrates critical for various cellular processes.
Biological function summary
AKT1 and AKT2 promote cell survival growth and metabolism. They do this by integrating signals from phosphatidylinositol (345)-trisphosphate (PIP3) and are often found as part of a larger signaling complex. These kinases are essential in moderating gene expression apoptosis and lipid metabolism. Their activity ensures a balance within the cell of life and death signals implicating them in processes like insulin response and nutrient availability.
Pathways
PI3K/AKT pathway is significant where AKT1 and AKT2 mediate responses to growth factors and extracellular signals. In this pathway these kinases interact with proteins like mTOR and GSK3β influencing cell growth and protein synthesis. Their role is also connected to the insulin signaling pathway impacting glucose uptake and metabolism and forming a critical hub integrating inputs from upstream signals.
AKT1 and AKT2 have strong associations with cancer and diabetes. Aberrant activation of these kinases has been observed in various cancers influencing tumor progression and resistance to conventional therapies. They are also implicated in type 2 diabetes through their involvement in insulin resistance and glucose homeostasis. In these contexts AKT1 and AKT2 activity affects other proteins such as BAD and FOXO transcription factors affecting cellular fate and metabolic processes.