Abcam, ab314433, Anti-pan Brd4 antibody [EPR25424-71] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal BRD4 antibody. Carrier free. Suitable for WB, IHC-P, Flow Cyt (Intra), ICC/IF, IP, ChIP-seq and reacts with Human, Mouse, Rat samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR25424-71,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human, Mouse, Rat,
Applications:ICC/IF, Flow Cyt (Intra), IP, ChIP-seq, IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Pan Brd4 also known as bromodomain-containing protein 4 belongs to the BET family. This protein often acts as an epigenetic reader recognizing acetylated lysine residues on histone tails. Brd4 has two bromodomains which contribute to its function in regulating gene expression by facilitating the assembly of transcriptional complexes. The Brd4 molecular weight varies depending on its isoforms with the short form around 75 kDa and the long form approximately 150 kDa. Brd4 is expressed widely across various tissues including the heart brain and testis.
Biological function summary
Brd4 plays an essential role in transcriptional regulation and control of gene expression. It interacts with multiple transcription factors and serves as a critical component of the super-enhancer complex where it aids in the recruitment of RNA polymerase II. Brd4 links the chromatin environment to transcription machinery facilitating transitions from chromatin condensation to active transcription. Furthermore Brd4 involvement extends to the regulation of cell cycle progression and mitotic bookmarking ensuring the retention of transcriptional memory through cell divisions.
Pathways
Brd4 integrates into vital cellular processes influencing inflammation and cancer pathways. In the context of the inflammatory pathway Brd4 associates with NF-kB a significant transcription factor in immune response regulation. For cancer pathways Brd4 influences MYC a well-recognized oncogene by driving its expression and subsequent cellular proliferation. These interactions highlight Brd4 as a prominent node in transcription regulation connecting to significant proteins involved in cellular growth and inflammation responses.
Brd4 significance emerges in cancer and cardiovascular diseases. In cancer such as acute myeloid leukemia Brd4 aberrantly regulates MYC resulting in uncontrolled cell proliferation and tumor growth. The connection here with MYC underpins Brd4's pivotal role in oncogenesis. In cardiovascular conditions Brd4 influences inflammatory gene expression contributing to pathologies like atherosclerosis. These roles make Brd4 a target for therapeutic development with inhibitors being investigated for their potential in treating cancers and inflammatory disorders associated with this protein.