Abcam, ab314531, Recombinant human Claudin 4 protein - Active (His tag)

Size: 20µg / 100µg
Recombinant human Claudin 4 protein - Active (His tag) is a Human Full Length protein, in the 1 to 209 aa range, expressed in Mammalian, < 1 EU/µg endotoxin level, suitable for WB, FuncS.
Key facts
Endotoxin level:< 1 EU/µg,
Expression system:Mammalian,
Tags:His tag C-Terminus,
Applications:WB, FuncSSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:Measured by its binding ability in a functional ELISA. AB314531 at 5 μg/mL can bind an Anti-CLDN4 recombinant antibody, the EC50 is 29.56-50.75 ng/mL.,
Accession:O14493,
Animal free:Yes,
Carrier free:No,
Species:Human,
Reconstitution:We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%.,
Storage buffer:pH: 7.4 - 8Constituents: 6% Trehalose, 0.14% PBS

Product details:
ab314531 is a VLP-CLDN4 protein complex.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, -80°C, Aliquoting information-Add glycerol to a final volume of 50% for extra stability and aliquot, Storage information-The product can be stored for up to 12 months

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Claudin-4 also known as CLDN4 is a protein essential for tight junctions which are critical in maintaining the permeability barrier of epithelial cells. The protein has a molecular mass of approximately 22 kDa. Claudin-4 expresses notably in epithelial cells of various tissues including the kidney lung and gastrointestinal tract. In addition to its presence in normal tissues claudin-4 can also exhibit heightened expression in certain cancerous tissues making it a subject of interest in oncology research.
Biological function summary
Claudin-4 plays a significant role in paracellular transport and cell polarity. By assembling into complexes with other claudins and tight junction proteins claudin-4 regulates the selective permeability and homeostasis across cell layers. It functions as a component of tight junction strands contributing to their barrier properties. Interaction with proteins such as occludin and ZO-1 further defines its role in tight junction structure and function.
Pathways
Claudin-4 participates in critical biological processes such as epithelial cell signaling and apoptosis regulation. It interfaces with the epithelial-to-mesenchymal transition (EMT) pathway where claudin-4 expression influences cellular adhesion and motility. Claudin-4 also interacts with related claudin proteins in these pathways contributing to structural arrangement and pathway signaling precision.
Claudin-4 has associations with several types of cancers including pancreatic and ovarian cancer. Its consistent overexpression in these conditions suggests its involvement in tumorigenesis and metastasis. In the context of ovarian cancer claudin-4 may interact with proteins such as mesothelin which plays a role in cell adhesion and tumor progression. Investigating claudin-4's involvement in cancer can provide insights into potential therapeutic targets and diagnostic markers.