Abcam, ab314532, Recombinant human Claudin 6 protein - Active (His tag)

Size: 20µg / 100µg
Recombinant human Claudin 6 protein - Active (His tag) is a Human Full Length protein, in the 1 to 220 aa range, expressed in Mammalian, < 1 EU/µg endotoxin level, suitable for WB, FuncS.
Key facts
Endotoxin level:< 1 EU/µg,
Expression system:Mammalian,
Tags:His tag C-Terminus,
Applications:FuncS, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:Measured by its binding ability in a functional ELISA. AB314532 at 10 μg/mL can bind an Anti-CLDN6/9 recombinant antibody, the EC50 is 1.501-2.035 ng/mL,
Accession:P56747,
Animal free:Yes,
Carrier free:No,
Species:Human,
Reconstitution:Reconstitute in water,
Storage buffer:pH: 7.4 - 8Constituents: 6% Trehalose, 0.87% Sodium chloride, 0.24% Tris

Product details:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20degC/-80degC. Our default final concentration of glycerol is 50%.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, -80°C, Appropriate long-term storage conditions--20°C, -80°C, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Claudin 6 also known as CLDN6 is a transmembrane protein belonging to the claudin family. This family is critical in forming tight junctions between cells which regulate the paracellular barrier functions of epithelia. The molecular mass of claudin 6 is approximately 23 kDa. Researchers have observed its expression mainly in embryonic tissues and certain cancer types. In normal adult tissues it shows limited expression indicating a specific role during early developmental stages.
Biological function summary
Claudin 6 participates in the formation and maintenance of tight junctions in epithelial cells. It is part of a larger complex that includes multiple claudins and other tight junction proteins like occludin and zonula occludens-1. This complex maintains cellular polarity and controls the flow of ions and molecules between cells which is essential for tissue homeostasis. Its expression pattern suggests a role in cell differentiation and proliferation during embryogenesis and tumorigenesis.
Pathways
Various studies indicate that claudin 6 is involved in the Wnt signaling pathway which is key in embryonic development and cell differentiation. This pathway interacts with several other proteins including β-catenin to mediate gene expression and cellular functions. Additionally claudin 6 contributes to pathways influencing the epithelial-mesenchymal transition (EMT) a process necessary for organ development and cancer metastasis. Through these pathways claudin 6 interacts with diverse proteins to modulate cell behavior.
Researchers have linked claudin 6 to certain cancers particularly ovarian and breast cancer. Its aberrant expression may contribute to tumor progression and invasiveness. Claudin 6 can promote changes in the tumor microenvironment by interacting with other cell junction proteins possibly influencing cancer cell proliferation and spread. Furthermore its altered expression is noted in immune disorders suggesting potential roles in pathogenesis through interactions with immune cells or signaling molecules like cytokines within the immune pathways.