Abcam, ab314535, Recombinant human Somatostatin Receptor 2 protein - Active (His tag)

Size: 20µg / 100µg
Recombinant human Somatostatin Receptor 2 protein - Active (His tag) is a Human Full Length protein, in the 1 to 369 aa range, expressed in Mammalian, < 1 EU/µg endotoxin level, suitable for WB, FuncS, BL.
Key facts
Endotoxin level:< 1 EU/µg,
Expression system:Mammalian,
Tags:His tag C-Terminus,
Applications:FuncS, WB, BLSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:Measured by its binding ability in a functional ELISA. AB314535 at 10 μg/mL can bind an Anti-SSTR2 recombinant antibody, the EC50 is 58.13-81.28 ng/mL.,
Accession:P30874,
Animal free:Yes,
Carrier free:No,
Species:Human,
Reconstitution:We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%.,
Storage buffer:pH: 7.4 - 8Constituents: 6% Trehalose, 0.14% PBS

Product details:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20degC/-80degC. Our default final concentration of glycerol is 50%.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, -80°C, Aliquoting information-Add glycerol to a final volume of 50% for extra stability and aliquot, Storage information-The product can be stored for up to 12 months

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The somatostatin receptor 2 (SSTR2) also known as SS2R serves as a G protein-coupled receptor with a molecular mass of approximately 40-60 kDa. SSTR2 specifically binds somatostatin a peptide hormone that regulates the endocrine system neurotransmission and cell proliferation via interaction with various somatostatin receptors. Scientists find SSTR2 expressed in high levels in the pituitary gland central nervous system gastrointestinal tract and pancreatic cells.
Biological function summary
Somatostatin receptor 2 mediates the inhibitory effects of somatostatin on cell growth and the release of other hormones. It functions as part of the receptor complex modulating intracellular signaling pathways. SSTR2 often interacts closely with other molecules like G proteins to inhibit adenylyl cyclase reducing cyclic AMP (cAMP) levels and influencing diverse cellular responses. The receptor's ability to influence such processes emphasizes its role in maintaining cellular and systemic homeostasis.
Pathways
Somatostatin receptor 2 plays an important role in the inhibitory signaling pathways related to growth hormone regulation and neurotransmitter release. SSTR2 actively participates in the somatostatin signaling pathway intimately interacting with proteins such as adenylate cyclase and phospholipase C. Another important pathway includes the regulation of the MAPK pathway serving to modulate cell proliferation and survival reiterating its regulatory function within endocrine signaling and cellular communication.
Somatostatin receptor 2 demonstrates significant involvement in neuroendocrine tumors and acromegaly. The overexpression of SSTR2 in neuroendocrine tumors provides a valuable target for diagnostic imaging and therapeutic interventions using somatostatin analogs. In acromegaly where excess growth hormone production occurs SSTR2's modulation of growth hormone release becomes important; it often works alongside growth hormone receptors and IGF-1 to limit abnormal growth. Therefore the receptor's targeting offers therapeutic potential and advances disease understanding.