Abcam, ab314544, Anti-USP48 antibody [EPR26175-11] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal USP48 antibody. Carrier free. Suitable for WB, IHC-P, Flow Cyt (Intra), ICC/IF and reacts with Human, Mouse, Rat samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR26175-11,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human, Mouse, Rat,
Applications:ICC/IF, IHC-P, WB, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab314544 is the carrier-free version of
ab314543
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
USP48 also known as Ubiquitin Specific Peptidase 48 acts as a deubiquitinating enzyme. It specifically removes ubiquitin from target proteins influencing protein stability and function. USP48 has a molecular mass of approximately 130 kDa. This enzyme expresses in various tissues with significant levels observed in the brain and immune cells. Researchers recognize its involvement in protein homeostasis through regulation of protein degradation pathways.
Biological function summary
Protein deubiquitination by USP48 affects cellular processes such as DNA repair transcription regulation and signal transduction. USP48 does not function as part of a stable complex but it associates transiently with substrates and other molecular partners during its activity. It has a role in maintaining genomic stability by modulating transcription factors and repair proteins through deubiquitination.
Pathways
One can find USP48 participating in the NF-kB signaling pathway. It stabilizes RelA an important NF-kB subunit by removing ubiquitin which influences the transcription of genes involved in immune response and inflammation. USP48 also interacts with proteins such as OTUB1 in these pathways contributing to the balance of protein ubiquitination and deubiquitination within the cell.
Research connections exist between USP48 and several cancers including glioblastoma due to its regulation of pathways that control cell survival and proliferation. USP48's interactions with NF-kB impact glioblastoma progression by altering the expression of genes that promote cell growth. Defective USP48 activity also associates with neurodegenerative disorders where its modulation of protein stability could contribute to neuron degeneration through inadequate DNA repair mechanisms.