Abcam, ab314554, Anti-NCOA4 antibody [EPR28511-12] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal NCOA4 antibody. Carrier free. Suitable for WB, IP and reacts with Human, Mouse, Rat samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR28511-12,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human, Mouse, Rat,
Applications:IP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab314554 is the carrier-free version of
ab314553
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The nuclear receptor coactivator 4 (NCOA4) also previously known as androgen receptor-associated protein 70 (ARA70) plays a critical role in cellular activities. It engages with nuclear receptors to enhance transcriptional activities. The molecular weight of NCOA4 is approximately 70 kDa. Scientists have observed its expression in various tissues including prostate and thyroid indicating its diverse functional capacity.
Biological function summary
NCOA4 modulates cellular iron homeostasis which is essential for cell survival and proliferation. It accomplishes this by mediating the autophagic degradation of ferritin a protein that stores iron through ferritinophagy. As part of the ferritinophagy complex NCOA4 works alongside other autophagic machinery to regulate iron bioavailability and mitigate oxidative stress maintaining cellular health and function.
Pathways
NCOA4 significantly influences the iron metabolism pathway an important component in maintaining iron balance within cells. The interaction with proteins such as ferritin highlights its role in facilitating iron release and utilization. Furthermore NCOA4 intersects with the autophagy pathway where it coordinates with autophagic proteins like LC3 to ensure proper iron recycling emphasizing its integration in cellular nutrient management.
Dysregulation of NCOA4 has links to neurodegenerative diseases like Parkinson’s disease where iron dysmetabolism plays a part in neuronal damage. Aberrant NCOA4 activity also relates to certain cancers as iron levels influence tumor growth. In these contexts NCOA4's interaction with proteins like p62 an important autophagy adaptor becomes critical highlighting its influence in disease progression and potential as a therapeutic target.