Abcam, ab314572, Human cleaved C-terminal GSDMD Antibody Pair - BSA and Azide free

Size: 10 x 96Tests
Human cleaved C-terminal GSDMD Antibody Pair - BSA and Azide free is a kit containing recombinant capture and detector antibodies in a carrier-free formulation for the measurement of Human cleaved C-terminal GSDMD.
Key facts
Reacts with:Human,
Assay type:ELISA set,
Range:15.6 - 1000 pg/mL,
Assay Platform:Reagents

Product details:
This antibody was developed in collaboration with Dr Feng Shao's lab, NIBS.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The pair can be used in variety of assays and platforms including but not limited to:
Sandwich ELISA
FRET/TR-FRET/HTRF
Meso Scale Discovery
Bead-based assays
AlphaLISA
/AlphaScreen
DELFIA
immunoassays
and Single Molecule Counting (SMC™) immunoassays
Multiplex
Our antibody pairs are supplied in a carrier-free format that is conjugation-ready:
Buffer free of BSA, sodium azide, and glycerol for higher conjugation efficiency.
Concentration of ~1 mg/ml as measured by the protein A280 method.
We can label antibodies for you: get in touch today to discuss how we can help accelerate your assay development with custom conjugation services.
Pairs are screened in biological samples, including plasma and serum, to ensure specificity in complex samples.
Please note:
The recommended antibody orientation is based on internal optimization in sandwich ELISA. Antibody orientation is assay dependent and needs to be optimized for each assay type.
The range provided for this antibody pair is based on initial sandwich ELISA validation data using recombinant protein. Performance and range of the antibody pair will depend on the specific characteristics of your assay, including standard protein selection.
We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibodies in other assays.
Antibody properties:
Capture antibody:
recombinant rabbit monoclonal (unconjugated) – 100 µg
Detector antibody:
recombinant rabbit monoclonal (unconjugated) - 100 µg
Concentration
: ~1 mg/ml
Storage buffer:
100% PBS
Liquid
Isotype:
Recombinant monoclonal antibodies offer several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free production
Meso Scale Discovery and MSD are registered trademarks of Meso Scale Diagnostics, LLC.
AlphaLISA, AlphaScreen, and DELFIA are registered trademarks of PerkinElmer, Inc.
Simoa is a registered trademark of Quanterix, Inc.
SMC is a registered trademark of Merck KGaA, Darmstadt, Germany.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Cleaved C-terminal GSDMD also known as cleaved gasdermin D is a protein involved in cellular processes influencing cell death. It is a fragment derived from gasdermin D (GSDMD) after cleavage which is facilitated by inflammatory caspases. This cleavage removes an inhibitory domain allowing the active N-terminal to initiate its functions. The molecular weight of GSDMD is approximately 53 kDa. Cleaved GSDMD is often detected through methods like Western blot particularly in immune cells such as macrophages and monocytes.
Biological function summary
Cleaved C-terminal GSDMD contributes significantly to the process of pyroptosis a form of programmed cell death that is inflammation-driven. This fragmented form is important for forming pores in the cell membrane leading to cellular lysis and the release of pro-inflammatory signals. Gasdermin D including its cleaved form is not known to be part of a large complex on its own but acts autonomously after activation by other proteins like caspase-1.
Pathways
Cleaved C-terminal GSDMD participates actively in the inflammatory response particularly in the inflammasome pathway. The protein is closely linked to caspases like caspase-1 that activate it leading to pyroptotic cell death. This pathway also intersects with other innate immune components such as the NLRP3 inflammasome to magnify inflammatory signals and recruit additional immune cells to sites of infection or damage.
Cleaved C-terminal GSDMD plays a role in conditions featuring excessive inflammation like septicemia and inflammatory bowel disease (IBD). In these diseases aberrant activation and overproduction of cleaved GSDMD can magnify tissue damage through uncontrolled cell death and inflammation. Also in septicemia GSDMD interacts with proteins like caspase-11 which can exacerbate inflammatory cytokine release and worsen the disease course.