Abcam, ab314578, Robo2 Antibody Pair - BSA and Azide free

Size: 10 x 96Tests
Robo2 Antibody Pair - BSA and Azide free is a kit containing recombinant capture and detector antibodies in a carrier-free formulation for the measurement of Robo2.
Key facts
Reacts with:Human, Mouse, Rat,
Assay type:ELISA set,
Range:31.25 - 2000 pg/mL,
Assay Platform:Reagents

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The pair can be used in variety of assays and platforms including but not limited to:
Sandwich ELISA
FRET/TR-FRET/HTRF
Meso Scale Discovery
Bead-based assays
AlphaLISA
/AlphaScreen
DELFIA
immunoassays
and Single Molecule Counting (SMC™) immunoassays
Multiplex
Our antibody pairs are supplied in a carrier-free format that is conjugation-ready:
Buffer free of BSA, sodium azide, and glycerol for higher conjugation efficiency.
Concentration of ~1 mg/ml as measured by the protein A280 method.
We can label antibodies for you: get in touch today to discuss how we can help accelerate your assay development with custom conjugation services.
Pairs are screened in biological samples, including plasma and serum, to ensure specificity in complex samples.
Please note:
The recommended antibody orientation is based on internal optimization in sandwich ELISA. Antibody orientation is assay dependent and needs to be optimized for each assay type.
The range provided for this antibody pair is based on initial sandwich ELISA validation data using recombinant protein. Performance and range of the antibody pair will depend on the specific characteristics of your assay, including standard protein selection.
We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibodies in other assays.
Antibody properties:
Capture antibody:
recombinant rabbit monoclonal (unconjugated) – 100 µg
Detector antibody:
recombinant rabbit monoclonal (unconjugated) - 100 µg
Concentration
: ~1 mg/ml
Storage buffer:
100% PBS
Liquid
Isotype:
Recombinant monoclonal antibodies offer several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free production
Meso Scale Discovery and MSD are registered trademarks of Meso Scale Diagnostics, LLC.
AlphaLISA, AlphaScreen, and DELFIA are registered trademarks of PerkinElmer, Inc.
Simoa is a registered trademark of Quanterix, Inc.
SMC is a registered trademark of Merck KGaA, Darmstadt, Germany.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Robo2 also known as Roundabout Homolog 2 is a protein with significant roles in axon guidance. This protein has a molecular mass of approximately 147 kDa and is a member of the Robo receptor family. It is primarily expressed in the nervous system including areas such as the spinal cord brain and olfactory bulb. Robo2 interacts with Slit proteins to mediate the repulsive guidance of axons which is fundamental for the formation of connections in the developing nervous system.
Biological function summary
Robo2 functions in the nervous system to guide axons and establish neuron networks. It plays a role in cellular processes like migration and axonal pathfinding ensuring proper connectivity. Robo2 operates as a receptor and does not form part of a larger complex by itself but it does have interactions with other molecules. By binding with Slit proteins Robo2 helps repel axons away from certain areas aiding in the clear mapping of axonal trajectories.
Pathways
Robo2 participates in axon guidance pathways including the Slit-Robo signaling pathway. This pathway is fundamental in nervous system development and is responsible for steering axonal growth direction. Within these pathways Robo2 displays interaction with proteins like neuron-specific Slit proteins which are secreted molecules helping to establish gradients that direct axon shrinkage or extension.
Robo2 is linked to conditions like congenital heart defects and urofacial syndrome. Abnormal expression or mutations in the Robo2 gene may disrupt normal axonal pathfinding leading to developmental issues. Robo2 has also shown associations with other proteins like Slit2 which together contribute to defective axon guidance when altered underlying certain neurodevelopmental disorders.