Abcam, ab314719, Human UNC5D ELISA Kit

Size: 1 x 96Tests
Human UNC5D ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human UNC5D in Human in Cell Culture Media, Biofluids samples.
Key facts
Detection method:Colorimetric,
Sample types:Cell culture supernatant, Plasma, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 0.085 ng/mL,
Range:0.085 - 20 ng/mL,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human UNC5D ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human UNC5D in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human UNC5D coated on a 96-well plate.
This ELISA kit shows no cross-reactivity with the following cytokines tested: human ANGPTL7, CD36, CLEC9a, CL-P1, Dectin-2, DLL4, DSCAM, EDIL3, ENPP-7, Enteropeptidase, FCRL3, FCRLB, FGF-3, FLRT1, FLRT2, GBA3, GDF-11, Glycoprotein V, Granzyme A, IGSF4B, IL-28 R alpha, Kynureninase, LAMA4, LRRC4, LRRTM3, NG2, NQO1, PCSK2, PILR-alpha, Plexin A4, POGLUT1, PRELP, Smad4, SOX15, SOX7, SOX9, Syntaxin 6, TROP-2, TSLP R.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
UNC5D also known as Unc-5 Netrin Receptor D is a transmembrane protein that is a member of the UNC5 receptor family. It has a molecular mass of approximately 110 kDa. UNC5D is primarily expressed in the human nervous system especially in developing neurons and is also found in tissues like the prostate breast and colon. This receptor serves an important role in cell guidance by interacting with netrin ligands acting as a dependence receptor that triggers apoptosis in the absence of netrin.
Biological function summary
UNC5D functions in axon guidance and neuronal migration. It plays a central role in the regulation of cell survival and apoptosis being part of a receptor complex that includes netrin-1. In the presence of netrin-1 UNC5D helps to mediate cell adhesion and guidance. The absence of its ligand however initiates programmed cell death making its presence significant in maintaining cellular homeostasis. This mechanism has implications in how neural networks are formed and maintained in the body.
Pathways
UNC5D influences both the apoptosis and axon guidance pathways. As a dependence receptor it is deeply involved in controlling cell fate through signaling pathways that govern cell survival and death. The interaction of UNC5D with netrin-1 is key in guiding neural development while also connecting with apoptotic pathways involving proteins such as DCC (Deleted in Colorectal Carcinoma). This relationship signifies its role in linking guidance cue responses with survival signals necessary for proper neural development.
Research implicates UNC5D in certain forms of cancer including prostate cancer and neuroblastoma. In these contexts the expression or suppression of UNC5D influences tumorigenesis. A loss of its pro-apoptotic function can promote cell survival in unwanted growths implicating netrin-1 as a significant interacting protein that disrupts this balance. Additionally altered UNC5D activity may affect neural disorders given its role in nervous system development and maintenance. As such it remains an important target for understanding and potentially treating certain cancers and neural conditions.