Abcam, ab314742, Anti-Rfx Cas13d (N-term) antibody [EPR23965-31] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal Rfx Cas13d antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra), IP and reacts with Transfected cell lysate - Ruminococcus flavefaciens, Transfected cell line - Ruminococcus flavefaciens samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR23965-31,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Ruminococcus flavefaciens,
Applications:ICC/IF, IHC-P, IP, WB, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab314742 is the carrier-free version of
ab314741
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Rfx Cas13d also known as C2c10 functions as a versatile RNA-targeted CRISPR-associated protein. Its primary mechanical action involves RNA-guided RNase activity facilitated by a guide RNA that directs the cleavage of specific RNA targets. This protein weighs approximately 930 amino acids and is expressed in various prokaryotic organisms. Engineered versions of Cas13d like Rfx Cas13d have been adapted for use in eukaryotic cells enabling targeted gene silencing and manipulation of RNA transcripts.
Biological function summary
Rfx Cas13d contributes to RNA processing and regulation playing a role in RNA interference mechanisms. It associates with RNA molecules and degrades them therefore regulating gene expression post-transcriptionally. Rfx Cas13d does not act alone; it forms part of a complex with guide RNA that determines its specificity. Its ability to cleave RNA makes it valuable for studying RNA function and interactions within cells offering insights into RNA-based regulation processes.
Pathways
Rfx Cas13d influences specific RNA regulatory pathways that control gene silencing and expression. It participates in pathways associated with RNA interference and endogenous processes similar to microRNA (miRNA) pathways. In these pathways it relates to proteins like AGO (Argonaute) which also play roles in RNA-induced silencing complexes. Through its RNA cleavage activity it helps manage RNA populations within cells ensuring correct gene expression regulation.
Rfx Cas13d holds potential for therapeutic applications especially in targeting RNA viruses and genetic diseases caused by RNA dysregulation. For example its ability to degrade viral RNA makes it a prospective tool in combating viral infections such as influenza. Also it may relate to disorders involving misregulated RNA species which in some cases connect to malfunctioning proteins thereby providing potential intervention points. Cas13d's action could help counteract diseases by restoring normal RNA regulation when linked to defective proteins or pathways.