Abcam, ab315228, Anti-Cytokeratin 1 + Cytokeratin 2e antibody [EPR27257-91]

Size: 20µL / 100µL / 1mL
Anti-Cytokeratin 1 + Cytokeratin 2e antibody [EPR27257-91] is a Rabbit Monoclonal antibody that is used in Cytokeratin 1 + Cytokeratin 2e Dot Blot, IHC-Fr, IHC-P, mIHC. Suitable for Human, Mouse samples. Cytokeratins are structural proteins found in epithelial cells, playing a crucial role in maintaining cell integrity and function. In cancer research, cytokeratins are used as biomarkers to differentiate between various types of carcinomas, aiding in tumor detection and classification. Their expression patterns can provide insights into the origin and progression of cancers, making them valuable in diagnostic and prognostic applications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR27257-91,
Isotype:IgG,
Carrier free:No,
Reacts with:Human, Mouse,
Applications:Dot, mIHC, IHC-P, IHC-FrSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Cytokeratin 1 (CK1) also known as keratin 1 is a type II cytokeratin with a molecular mass of approximately 66 kilodaltons. Alongside Cytokeratin 2e (CK2e) these proteins form a part of the keratin family which are structural proteins in epithelial cells. Specifically CK1 and CK2e are expressed in the suprabasal cells of stratified epithelia like in the epidermis. These cytokeratins create filaments providing mechanical support and protecting epithelial cells from damage.
Biological function summary
Cytokeratins 1 and 2e contribute to the overall stability and rigidity of the epidermal layer. They participate in the assembly of the keratin intermediate filament complex which maintains the structural integrity of cells subjected to stress. As part of this filament network CK1 and CK2e interact with other cytokeratins such as CK10 forming a lattice structure essential for the skin barrier function. This interaction allows cells to withstand mechanical stress and abrasion important for maintaining skin health.
Pathways
CK1 and CK2e have roles in the regulation of cell proliferation and differentiation pathways. These proteins integrate into the epidermal differentiation complex influencing pathways like the Notch signaling pathway. The interaction with Notch signaling components helps regulate epidermal homeostasis by controlling the balance between proliferation and differentiation. CK1 and CK2e work alongside CK10 and other keratins promoting orderly differentiation and limiting excessive cell division.
CK1 and CK2e relate significantly to skin disorders particularly ichthyosis and epidermolytic hyperkeratosis. Mutations or dysregulation of these cytokeratins can disrupt the structural integrity of skin cells leading to hyperproliferative disorders. Cytokeratin 1 displays connections to proteins involved in skin barrier maintenance such as filaggrin with disruptions in these associations contributing to disease phenotypes. Understanding these interactions aids in uncovering the pathological mechanisms driving these skin disorders.