Abcam, ab315235, Anti-ITM2B antibody [EPR27849-47] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal ITM2B antibody. Carrier free. Suitable for Flow Cyt (Intra), WB and reacts with Mouse, Rat, Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR27849-47,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:Flow Cyt (Intra), WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab315235 is the carrier-free version of
ab315234
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ITM2B also known as Integral Membrane Protein 2B or BRI2 is a transmembrane protein weighing approximately 30 kDa. This protein is expressed widely in human tissues showing particularly high levels in the central nervous system and brain. ITM2B functions mechanically by playing a role in protein processing and trafficking owing to its transmembrane nature. With an integral role in cellular functions ITM2B interacts with various cellular components associated with membrane-related activities.
Biological function summary
ITM2B engages in several vital cellular processes such as regulation of amyloid precursor protein (APP) processing. ITM2B forms part of a complex with other proteins involved in APP cleavage which is significant for maintaining cell homeostasis. It also influences neuronal functions through its effects on synaptic plasticity and could impact cognitive abilities. The protein's presence in brain regions ties ITM2B closely to neural activities where protein interactions are essential for cellular communication.
Pathways
ITM2B exerts influence on critical processes in amyloidogenic pathways contributing to the modulation of amyloid-beta production. This protein directly interacts with other players like APP and presenilin 1 within this pathway affecting the levels of amyloids and ultimately impacting neural integrity. Additionally ITM2B participates in Wnt signaling pathways linking it to cellular differentiation and proliferation where it modulates interactions with proteins that convey Wnt signals such as beta-catenin.
ITM2B is directly linked to familial British dementia and familial Danish dementia both neurodegenerative conditions. These diseases involve mutations in the ITM2B gene leading to abnormal protein aggregates that disrupt normal brain function. ITM2B's relationship with amyloid-beta and presenilin 1 also connects it to Alzheimer's disease pathogenesis where these proteins play roles in amyloid plaque formation a hallmark of Alzheimer's. Studies focus on targeting ITM2B interactions and processing to develop therapies for these conditions highlighting its potential as a therapeutic target.