Abcam, ab315260, Alexa Fluor® 488 Anti-UBE3A antibody [EPR23077-14]

Size: 100µL
Rabbit Recombinant Monoclonal UBE3A antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF and reacts with Human, Mouse samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR23077-14,
Isotype:IgG,
Conjugation:Alexa Fluor® 488,
Excitation/Emission:Ex: 495nm, Em: 519nm,
Carrier free:No,
Reacts with:Human, Mouse,
Applications:ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle, Store in the dark

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
UBE3A also known as ubiquitin protein ligase E3A is an enzyme with a molecular mass of approximately 100 kDa. It plays a central role in the ubiquitination process by adding ubiquitin to specific substrate proteins. UBE3A is found in many tissues but has high expression levels in the brain especially in neurons. The proper function of UBE3A requires its interaction with the ubiquitin-conjugating enzyme E2 marking target proteins for proteasomal degradation or other cellular processes.
Biological function summary
The role of UBE3A is connected to protein homeostasis and turnover. It is part of the ubiquitin-proteasome system which regulates the degradation of damaged or unneeded proteins. UBE3A does not work alone; it functions as part of a larger E3 ligase complex. This complex selectively identifies and modifies proteins determining their fate within the cell. The precision of UBE3A in these processes is necessary for normal neural development and synaptic plasticity.
Pathways
UBE3A is involved in the synaptic transmission and neuronal signaling pathways. It interacts with proteins such as HERC2 and RAD18 which are involved in DNA damage response and repair. UBE3A's involvement in these pathways highlights its importance in maintaining neuronal health. This protein’s activity influences downstream signaling events affecting processes like synaptic function and neuronal communication.
Disruptions in UBE3A activity link to Angelman syndrome and autism spectrum disorders. Angelman syndrome results from the loss of function of the maternal UBE3A gene leading to severe neurological impairments. UBE3A's relationship with proteins like MECP2 involved in gene regulation suggests a broader role in neurodevelopmental disorders. Research continues to explore how UBE3A and its interaction partners contribute to the underlying mechanisms of these diseases.