Abcam, ab315462, Anti-MBD4/MED1 antibody [EPR28263-16]

Size: 20µL / 100µL / 1mL
Knockout Tested Rabbit Recombinant Monoclonal MBD4/MED1 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR28263-16,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The MBD4 protein also known as MED1 is essential in DNA repair and gene regulation. MBD4 contains a methyl-CpG-binding domain allowing it to bind specifically to methylated DNA. The protein has a molecular weight of about 63 kDa. It is expressed in various tissues with higher levels in bone marrow and lymphoid tissues. MBD4 can recognize and excise mismatched T/G base pairs at methylated CpG sites which is important for maintaining genomic stability.
Biological function summary
MBD4 participates in the protection of genome integrity. It is involved in the DNA demethylation process ensuring proper gene expression patterns. MBD4 functions as part of complex DNA repair machinery. By working with other repair proteins MBD4 facilitates the correction of errors arising during DNA replication and demethylation reducing the risk of mutation accumulation and genomic instability.
Pathways
MBD4 plays a significant role in the base excision repair (BER) pathway. This pathway is key for correcting DNA damage caused by deamination and other forms of DNA alteration. In the BER pathway MBD4 cooperates with proteins like DNA glycosylases to identify and remove incorrect bases from the DNA strand. MBD4 also interacts with transcriptional regulation pathways influencing how genes are expressed in response to changes in the epigenetic landscape.
The malfunction of MBD4 links to colorectal cancer and other forms of cancer. Deficiency or mutations in the MBD4 gene increase the likelihood of mutation accumulation leading to tumorigenesis. MBD4's interactions with other proteins involved in DNA repair such as MLH1 highlight its role in maintaining DNA integrity. Genetic instability from impaired MBD4 function could trigger or exacerbate progression of cancerous conditions.