Abcam, ab315464, Anti-KMT2A / MLL + KMT2B / MLL4 antibody [EPR28282-16] - C-terminal

Size: 20µL / 100µL / 1mL
Knockout Tested Rabbit Recombinant Monoclonal KMT2A / MLL antibody. Suitable for WB and reacts with Human, Mouse, Rat, Recombinant fragment - Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR28282-16,
Isotype:IgG,
Carrier free:No,
Reacts with:Human, Mouse, Rat,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:This antibody has cross reaction with human KMT2B.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
KMT2A also known as MLL and KMT2B also called MLL4 are essential histone methyltransferases. These proteins have a molecular mass of approximately 430-431 kDa. KMT2A is expressed mainly in the cell nucleus and functions as a transcriptional co-regulator. MLL4 is similar and also found in various tissues with considerable expression in the developing brain. Both KMT2A and MLL4 perform important roles in the modulation of chromatin structure through methylation of histone H3 at lysine 4 influencing gene expression.
Biological function summary
KMT2A and MLL4 participate in transcriptional regulation. They are components of large protein complexes that include WDR5 RBBP5 and ASH2L which facilitate chromatin remodeling and gene activation. By modifying chromatin states these proteins influence the expression of genes involved in development and differentiation. They are particularly active during early embryonic development playing significant roles in hematopoiesis and neuronal lineage specification ensuring proper gene activation patterns necessary for these processes.
Pathways
These proteins significantly contribute to the regulation of gene expression pathways such as the Wnt signaling and the HOX gene regulatory network. Their activity within the Wnt signaling pathway helps control cell fate decisions and cellular proliferation. KMT2A and MLL4 interact with other pathway-related proteins like β-catenin facilitating the transcription of target genes. In the context of the HOX gene regulatory network these proteins ensure proper developmental patterns by interacting with other transcription factors to establish body axis formation.
Malfunctioning of KMT2A and MLL4 links to various conditions like acute leukemia and Kabuki syndrome. In leukemia chromosomal translocations involving KMT2A lead to fusion proteins that disrupt normal gene regulation contributing to the malignancy. These fusion proteins often partner with other proteins such as AF4 ENL or ELL to sustain the leukemogenic process. Similarly mutations in MLL4 associate with Kabuki syndrome a disorder characterized by intellectual disability and growth delay involving its connection with proteins like KDM6A which regulate chromatin structure and function.