Abcam, ab315789, Mouse Fibrinogen ELISA Kit- Intracellular

Size: 1 x 96Tests
Mouse Fibrinogen ELISA Kit- Intracellular is a single-wash 90-min Simplestep used to quantify Mouse Fibrinogen with a sensitivity of 67 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Tissue Extracts, Cell Lysate,
Reacts with:Mouse, Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 67 pg/mL,
Range:281.25 - 18000 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Mouse Fibrinogen SimpleStep ELISA
kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Fibrinogen protein in Tissue extracts, Cell Lysate. Quantitate Mouse Fibrinogen with 67 pg/ml sensitivity.
SimpleStep ELISA
technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA
plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA
protocol summary in the image section for further details. Our SimpleStep ELISA
technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA
microplate (
ab203359
) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA
CROSS REACTIVITY
The following recombinant proteins were prepared at 50 ng/mL and 8,000 pg/mL and assayed for cross reactivity and no cross reactivity was observed: Human Fibrinogen alpha, Human Fibrinogen beta, Human Fibrinogen gamma, Human Factor XII, Human Plasmin, Human Thrombin, Rat Fibrinogen
The following recombinant proteins were prepared at 50 ng/mL and 8,000 pg/mL and assayed for cross reactivity.
Human D-Dimer: 27% cross-reactivity
SPECIES REACTIVITY
This kit recognizes human and mouse Fibrinogen protein.
Native purified human Fibrinogen was prepared at 18 ng/mL and assayed for cross reactivity. 145% cross reactivity was observed.
Other species reactivity was determined by measuring plasma – EDTA samples of various species, interpolating the protein concentrations from the mouse standard curve, and expressing the interpolated concentrations as a percentage of the protein concentration in mouse plasma assayed at the same dilution.
Reactivity was determined for the following species: Human (147%), Monkey (9%)
No signal was observed for the following species: Rat, Cow
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C