Abcam, ab315846, Alexa Fluor® 647 Anti-ENPP1/PC1 antibody [EPR22262-22]

Size: 100µL
Rabbit Recombinant Monoclonal ENPP1/PC1 antibody - conjugated to Alexa Fluor® 647. Suitable for IHC-P, ICC/IF and reacts with Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR22262-22,
Isotype:IgG,
Conjugation:Alexa Fluor® 647,
Excitation/Emission:Ex: 650nm, Em: 665nm,
Carrier free:No,
Reacts with:Human,
Applications:IHC-P, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle, Store in the dark

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ENPP1 also known as PC-1 is a type II transmembrane glycoprotein with a molecular weight of approximately 130 kDa. It is expressed in various tissues including the liver kidney and bone. ENPP1 exhibits the enzymatic activity of nucleotide pyrophosphatase/phosphodiesterase which hydrolyzes ATP to AMP and diphosphate. This activity is important for the regulation of extracellular nucleotide levels and mineralization processes. Researchers often utilize the ENPP1 assay to study its function and activity.
Biological function summary
ENPP1 significantly influences bone mineralization and soft tissue calcification. It interacts with proteins like ectonucleoside triphosphate diphosphohydrolase-1 (ENTPD1) impacting cellular uptake of calcified materials. The protein is also involved in the regulatory networks responsible for controlling insulin signaling impacting metabolic processes. ENPP1 may form complexes with other proteins which can finely tune its biological activity and effects.
Pathways
ENPP1 plays a role in the insulin signaling pathway and the pyrophosphate pathway. Via its participation in insulin signaling ENPP1 regulates glucose metabolism and influences insulin sensitivity. It affects pathways related to mineral metabolism where it interacts with proteins like ENPP3. By regulating extracellular pyrophosphate levels ENPP1 contributes to inorganic phosphate homeostasis and influences skeletal development and maintenance.
ENPP1 has associations with type 2 diabetes and generalized arterial calcification of infancy (GACI). Its role in type 2 diabetes relates to its ability to inhibit insulin receptor function therefore contributing to insulin resistance. ENPP1 also connects to proteins involved in vascular calcification such as matrix Gla protein (MGP) in the context of GACI. These associations highlight the importance of ENPP1 in metabolic and calcification disorders.