Abcam, ab315896, Anti-NOTUM antibody [EPR27981-76] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal NOTUM antibody. Carrier free. Suitable for WB, IP and reacts with Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR27981-76,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:IP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab315896 is the carrier-free version of
ab315895
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
NOTUM is a lipase-like enzyme that functions mechanically to remove lipid modifications specifically palmitoleate from the Wnt proteins. It is alternatively known as palmitoleoyl-protein carboxylesterase. The molecular mass of NOTUM is approximately 53 kDa. NOTUM is expressed mainly in tissues such as the liver kidneys and gut where it plays a role in regulating signaling pathways.
Biological function summary
NOTUM inhibits the Wnt signaling pathway by cleaving the lipid moiety attached to Wnt proteins making it inactive. NOTUM acts independently and is not part of larger protein complexes. This regulation of Wnt signaling is important for maintaining cellular processes such as cell proliferation differentiation and migration especially during development and tissue homeostasis.
Pathways
NOTUM primarily influences the Wnt signaling pathway. This pathway is significant for embryonic development and adult homeostasis. By inactivating Wnt proteins NOTUM indirectly affects other pathway components like β-catenin which is an important mediator in the Wnt pathway. NOTUM ensures the balance of Wnt signaling preventing overactivation that could lead to abnormal cell growth.
NOTUM's role in regulating Wnt signaling links it to conditions such as colorectal cancer and osteoporosis. In cancers overexpression of NOTUM can contribute to Wnt pathway dysregulation while its inhibition may present a therapeutic angle. In osteoporosis alterations in NOTUM activity can affect bone formation processes through its influence on Wnt-related proteins like LRP5/6. Understanding NOTUM's interactions in these diseases may provide insights into novel treatment strategies.