Abcam, ab315946, Anti-LANCL2 antibody [EPR27973-111] - BSA and Azide free (Detector)

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal LANCL2 antibody. Carrier free. Suitable for sELISA and reacts with Human, Mouse samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR27973-111,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human, Mouse,
Applications:sELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab315946 is a BSA and Azide free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human/Mouse LANCL2. The recommended pair for sandwich ELISA is:
Capture:
ab315945
, Human/Mouse LANCL2 Capture Antibody (unconjugated)
Detector: ab315946, Human/Mouse LANCL2 Detector Antibody (unconjugated)
The reference range value is 0.039-10 ng/mL.
The recommended antibody orientation is based on internal optimization for ELISA based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
LANCL2 also known as Lanthionine Synthetase C-Like 2 is a protein with a molecular weight of approximately 44 kDa. It belongs to the LANCL protein family which also includes LANCL1 and LANCL3. LANCL2 is expressed in various tissues with significant expression observed in adipose tissue liver and skeletal muscle. Its intracellular location suggests a role in cellular processes that require close interaction with intracellular signaling pathways.
Biological function summary
LANCL2 functions in regulatory mechanisms related to metabolic processes. It does not exist in isolation; instead it interacts with other proteins as part of a multiprotein complex. LANCL2 modulates the signaling pathway involving the peroxisome proliferator-activated receptor gamma (PPARγ) and plays a role in modulating glucose and lipid metabolism. Its activity suggests involvement in maintaining cellular energy balance and homeostasis.
Pathways
LANCL2 is integrated into metabolic pathways with significant involvement in both the PPAR signaling pathway and the insulin signaling pathway. These pathways highlight its importance in glucose homeostasis and energy metabolism. LANCL2 interacts with proteins such as AKT an important player in the insulin signaling pathway contributing to the regulation of glucose uptake and lipid metabolism.
LANCL2 has been connected to metabolic diseases particularly type 2 diabetes and obesity. Changes in LANCL2 expression or function can impact glucose and lipid regulation contributing to these conditions. LANCL2's role in these disorders often involves its interaction with proteins like PPARγ which also influences lipid storage and insulin sensitivity. Understanding LANCL2's role in these diseases could provide insights into novel therapeutic approaches.