Abcam, ab315964, Anti-SARS-CoV2 nsp12 antibody [EPR24837-213]

Size: 20µL / 100µL / 1mL
Rabbit Recombinant Monoclonal R1AB antibody. Suitable for WB, ICC/IF, IP, IHC-P, Flow Cyt and reacts with SARS-CoV-2 samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR24837-213,
Isotype:IgG,
Carrier free:No,
Reacts with:SARS-CoV-2,
Applications:IP, WB, IHC-P, Flow Cyt, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SARS-CoV2 nsp12 also known as RNA-dependent RNA polymerase (RdRp) plays a central role in the viral replication cycle of the SARS-CoV2 virus. This enzyme has a molecular mass of approximately 106 kDa. It carries out its activities inside the host cell where the virus replicates such as in lung epithelial cells. Mechanically nsp12 facilitates the production of new viral RNA strands by copying the viral RNA genome allowing the virus to proliferate within the host.
Biological function summary
RNA-dependent RNA polymerase coordinates with other non-structural proteins to form the replication-transcription complex. Within this complex vital functions include the catalysis of viral RNA synthesis. The enzyme interacts with cofactors like nsp7 and nsp8 to enhance its polymerase activity and ensure efficient replication of viral RNA. These interactions are important to the viral life cycle making nsp12 an important player in SARS-CoV2 biology.
Pathways
The replication and transcription activities mediated by nsp12 integrate into the viral RNA synthesis pathway. This pathway is essential for the synthesis of subgenomic RNA and ultimately the production of structural proteins necessary for virion assembly. Nsp12 works closely with proteins such as nsp7 and nsp8 forming a replication complex to execute these functions. These interactions ensure the processive synthesis of RNA and are highly conserved across coronaviruses.
Nsp12 closely associates with COVID-19 the disease caused by SARS-CoV2. This protein's role in viral replication makes it a target for antiviral drug development as inhibiting nsp12 could block viral replication and reduce viral load in infected individuals. Remdesivir an antiviral medication targets this protein to impair its function. Mutation or dysfunction in nsp12 combined with its interaction with other viral proteins directly correlates with viral pathogenicity and disease severity highlighting its significance in the context of SARS-CoV2 infection.