Abcam, ab316075, Recombinant Human M-CSF/CSF-1 (Active) protein

Size: 100µg / 500µg
Recombinant Human M-CSF/CSF-1 (Active) protein is a Human Fragment protein, in the 33 to 190 aa range, expressed in HEK 293 cells, with >95%, < 1 EU/µg endotoxin level.
Key facts
Purity:>95% HPLC,
Endotoxin level:< 1 EU/µg,
Expression system:HEK 293 cells,
Tags:Tag free,
Biologically active:Yes,
Biological activity:Measured in a cell proliferation assay using RAW264.7 cells.The ED50-for this effect is 2.5 - 7.0 ng/mL.,
Accession:P09603,
Animal free:No,
Carrier free:No,
Species:Human,
Reconstitution:Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.,
Storage buffer:Constituents: PBS, 8% Trehalose

Properties and Storage Information:
Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage duration-A few days, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C|-80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
M-CSF or macrophage colony-stimulating factor is a cytokine involved in the regulation of macrophage production. Alternative names for M-CSF include CSF-1 and colony-stimulating factor 1. The M-CSF protein has a molecular mass of approximately 18 to 22 kDa. M-CSF is mainly expressed in various tissues including the placenta kidney and bone marrow. It functions as a homodimer important for the survival proliferation and differentiation of mononuclear phagocyte lineage cells.
Biological function summary
M-CSF influences the production differentiation and function of macrophages and osteoclasts. It is not part of a large complex but interacts with its receptor the CSF1R (colony-stimulating factor 1 receptor) on the surface of target cells. This interaction promotes the survival and proliferation of the target cells playing significant roles in immune response and bone homeostasis by regulating osteoclast development.
Pathways
The interaction of M-CSF with its receptor is central to several biological pathways notably the immune system pathway and bone resorption pathway. Within these pathways the binding of M-CSF to CSF1R activates downstream signaling cascades such as the PI3K/AKT and MAPK pathways importantly affecting cell survival and differentiation. The M-CSF pathway intersects with other cytokines and factors like GM-CSF (granulocyte-macrophage colony-stimulating factor) which also regulate immune cell dynamics.
Dysregulation of M-CSF levels is implicated in conditions such as osteoporosis and certain cancers. In osteoporosis M-CSF’s role in osteoclast development links to increased bone resorption leading to bone loss. In cancers M-CSF overexpression may facilitate tumor-associated macrophage infiltration therefore supporting tumor progression. The interaction with CSF1R is significant as it serves as a potential target for therapeutic strategies aimed at modulating macrophage activity in these diseases.