Abcam, ab316111, Anti-SPG3A/ATL1 antibody [EPR28057-64]

Size: 20µL / 100µL / 1mL
Rabbit Recombinant Monoclonal SPG3A/ATL1 antibody. Suitable for WB, IHC-P, ICC/IF, IHC-Fr, Flow Cyt (Intra), IP and reacts with Transfected cell lysate - Human, Human, Mouse, Rat samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR28057-64,
Isotype:IgG,
Carrier free:No,
Reacts with:Human, Mouse, Rat,
Applications:WB, IP, IHC-Fr, Flow Cyt (Intra), IHC-P, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SPG3A also known as Atlastin-1 or ATL1 is a GTPase enzyme with a molecular mass of approximately 64 kDa. This protein is highly important in the mechanical process of membrane trafficking and the maintenance of the endoplasmic reticulum (ER) network. Atlastin-1 is expressed predominantly in neurons especially in the cerebral cortex and the hippocampus where it plays a role in neural function.
Biological function summary
Atlastin-1 is involved in the fusion of ER membranes contributing to the network's tubular structure. The protein facilitates the dimerization and fusion of ER membranes a function that is fundamental for creating a continuous and smooth ER network. While it does not form part of a large protein complex its role in membrane dynamics involves interaction with other proteins that maintain ER structure and function.
Pathways
Atlastin-1 participates in the ER and Golgi-related pathways essential for protein trafficking and cellular homeostasis. It has significant interactions in these pathways with proteins like REEP1 and spastin both of which assist in maintaining the dynamic structure of the ER. These interactions underline Atlastin-1's contribution to vesicle transport and membrane fusion processes.
Mutations in the Atlastin-1 gene can lead to hereditary spastic paraplegia (HSP) a group of inherited disorders characterized by weakness and stiffness of the legs. This protein interacts in the context of HSP with proteins like spastin both of which are implicated in similar neuronal membrane dynamics disorders. Another condition linked to Atlastin-1 dysregulation is neuropathy where the protein's failure to maintain ER integrity can contribute to neuronal degradation.