Abcam, ab316188, Anti-IgA1 antibody [EPR26775-27] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal IGHA1 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Purified native protein - Human, Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR26775-27,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab316188 is the carrirer-free version of
ab316187
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
IgA1 also known as Immunoglobulin A1 is an important component of the immune system. This protein mainly exists in mucous membranes where it plays an important role in immune function. IgA1 has a mass of approximately 160 kDa and is expressed in various tissues including the respiratory and gastrointestinal tracts. IgA1 is a subclass of immunoglobulin A (IgA) and is characterized by its heavy chain constant region which distinguishes it from other immunoglobulin subclasses.
Biological function summary
IgA1 acts as a first line of defense in the immune system by binding to pathogens and neutralizing them. It exists as a monomer or forms a dimer with the help of the J chain which stabilizes its dimeric form. IgA1 also interacts with the polymeric immunoglobulin receptor (pIgR) for transportation across epithelial cells. This transport allows IgA1 to be present in secretory fluids such as saliva and breast milk providing an important protective function.
Pathways
IgA1 interacts with several pathways involved in immune response and mucosal immunity. One critical pathway is the mucosal antibody response where IgA1 plays a role in preventing infection at mucosal surfaces. The IgA1 protein activates the alternative complement pathway by binding to complement protein C3 which enhances the removal of pathogens. Furthermore IgA1 is associated with the J chain and pIgR in the process of IgA transcytosis across epithelial barriers.
IgA1 has strong connections to IgA nephropathy and celiac disease. In IgA nephropathy IgA1 deposits in glomeruli leading to inflammation and kidney damage. The increased serum levels of IgA1 often with aberrant glycosylation contribute to disease development. In celiac disease IgA1 reacts with tissue transglutaminase causing an immune response that damages the small intestine's lining. In both disorders the interactions between IgA1 and other proteins such as tissue transglutaminase in celiac disease highlight its involvement in pathological processes.