Abcam, ab316284, Anti-Hepatitis B Virus Core Antigen antibody [EPR28251-34] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal CAPSD antibody. Carrier free. Suitable for I-ELISA, Flow Cyt (Intra), ICC/IF, IHC-P, WB and reacts with Synthetic peptide - Hepatitis B virus, Transfected cell line - Hepatitis B virus, Transfected cell lysate - Hepatitis B virus samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR28251-34,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Hepatitis B virus,
Applications:ICC/IF, I-ELISA, WB, Flow Cyt (Intra), IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab316284 is the carrirer-free version of
ab316283
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The Hepatitis B Virus Core Antigen also known as HBV core antigen or Hep B core is an important component of the hepatitis B virus (HBV). This antigen often referred to simply as 'core' forms the nucleocapsid of the virus. The HBV core antigen consists of a protein structure with a mass of approximately 21 kilodaltons. It is primarily expressed within the hepatocytes the liver cells where it plays a pivotal role in viral replication and assembly. The HBV core antigen is also referred to in studies as HBV protein or B core.
Biological function summary
The hepatitis B core antigen is integral for the assembly of the viral capsid which protects the viral genome during infection. It functions as part of a larger complex with other viral proteins including the pre-core and envelope proteins to facilitate viral lifecycle processes. This antigen allows for the encapsidation of viral RNA and serves as a scaffold for reverse transcription of the viral genome ensuring replication fidelity and successful infection spread.
Pathways
The hepatitis B core antigen is essential in the viral replication pathway and the HBV lifecycle pathway. It interacts closely with HBV polymerase during the packaging of viral genome into the capsid. These interactions are important for the transformation of RNA into DNA a critical step in viral maturation. The antigen is related to the HBx protein through these pathways; the HBx protein modulates viral replication and can influence the pathogenesis of the virus.
The hepatitis B core antigen is strongly linked to chronic hepatitis B infection and liver diseases such as cirrhosis and hepatocellular carcinoma. The persistence of the core antigen in infected hepatocytes signifies ongoing viral replication which can lead to liver inflammation and damage. The interaction of the core antigen with immune system components such as anti-HBc antibodies assists in the diagnosis and monitoring of disease progression. It connects to the envelope proteins during the disease process which are important in immune system evasion and chronic infection establishment.