Abcam, ab316334, Anti-pan NOS antibody [RM1126]

Size: 20µL / 100µL / 1mL
Rabbit Recombinant Multiclonal eNOS antibody. Suitable for Dot, IHC-P and reacts with Recombinant full length protein - Human, Human, Transfected cell line - Human samples.
Key facts
Host species:Rabbit,
Clonality:Multiclonal,
Clone number:RM1126,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IHC-P, DotSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:This product was produced with the following immunogens:The exact immunogen used to generate this antibody is proprietary information.The exact immunogen used to generate this antibody is proprietary information.The exact immunogen used to generate this antibody is proprietary information.

Product details:
What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:
- The sensitivity of polyclonal antibodies by recognising multiple epitopes
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
View our range of
recombinant multiclonal antibodies
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Nitric Oxide Synthases (NOS) are enzymes responsible for the production of nitric oxide (NO) from L-arginine. NOS proteins exist in three main isoforms: neuronal NOS (nNOS or NOS1) inducible NOS (iNOS or NOS2) and endothelial NOS (eNOS or NOS3). They range in molecular mass between 130 and 160 kDa. Pan NOS refers to any antibody or inhibitor that targets all three NOS isoforms. These enzymes are expressed in various tissues with nNOS mainly in neural tissue iNOS in immune cells and eNOS in endothelial cells. Each isoform plays a unique role in its respective location.
Biological function summary
Nitric oxide acts as a signaling molecule with diverse functions within the body. It does not bind into large permanent complexes but interacts transiently with various proteins to relay signals. In the nervous system nNOS-generated NO modulates neurotransmission and contributes to synaptic plasticity. In blood vessels eNOS-derived NO plays an important role in vasodilation and blood pressure regulation. iNOS often gets activated during immune responses producing larger amounts of NO to fight pathogens.
Pathways
Nitric oxide produced by NOS enzymes participates in significant signaling cascades. The L-arginine-NO-cGMP pathway is one of the primary routes involving cyclic guanosine monophosphate (cGMP) as a secondary messenger. The NO-cGMP pathway interacts with proteins like soluble guanylyl cyclase which then modulates various physiological responses like vasodilation and neurotransmission. NOS activity also interplays with oxidative stress pathways interacting with reactive oxygen species (ROS) and affecting cellular responses like inflammation and apoptosis.
Aberrations in NOS activity relate to cardiovascular disorders and neurodegenerative diseases. For instance dysregulated eNOS activity can contribute to hypertension due to impaired vasodilation. In neurodegenerative conditions such as Alzheimer's disease abnormal nNOS activity may exacerbate neuronal damage through excessive NO production. Furthermore iNOS expression links to inflammatory conditions where excess NO might damage tissues. NOS activity can also intersect with proteins like amyloid-beta in Alzheimer's amplifying neurodegenerative processes.