Abcam, ab316490, Recombinant Human IL-2RB Protein Standard (His tag)

Size: 10µg
Recombinant Human IL-2RB Protein Standard (His tag) is a Human Fragment protein, expressed in HEK 293 cells, with >80%, suitable for SDS-PAGE, sELISA.
Key facts
Purity:>80% SDS-PAGE,
Expression system:HEK 293 cells,
Tags:His tag C-Terminus,
Applications:sELISA, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:P14784,
Animal free:Yes,
Carrier free:No,
Species:Human,
Storage buffer:pH: 7.3 - 7.5Constituents: 2.922% Sodium chloride, 0.64107% disodium;hydrogen phosphate;dodecahydrate, 0.02858% Potassium phosphate monobasic

Product details:
While the standard is the same as the one provided in the corresponding SimpleStep ELISA Kit, it cannot be treated as the consumable provided with our SimpleStep ELISA Kit due to differences in its concentration calibration.
Abcam guarantee that this protein standard is suitable for use in a sandwich ELISA. Individual results may vary due to differences in technique, laboratory equipment, buffers, and other experimental factors. The detection range provided for this protein standard is based on initial sandwich ELISA validation data.
The protein concentration is the concentration after validation on our sandwich ELISA platform. This Standard protein is guaranteed to work with our Capture and Detector antibodies in sELISA. Please contact our Scientific Support team to know which antibody pair is suitable for this protein.

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The soluble IL2 Receptor beta also known as IL2Rβ or CD122 is a component of the interleukin-2 beta receptor complex. It has a molecular mass of approximately 55 kDa. IL2Rβ exists in both membrane-bound and soluble forms. This receptor is expressed on various immune cells including natural killer cells and some T lymphocytes. The soluble form of IL2Rβ can be detected in body fluids and often increases in response to immune activation.
Biological function summary
The soluble IL2 Receptor beta plays an important role in immune system regulation by modulating the activity of its ligand the IL2 protein. It forms part of the high-affinity IL-2 receptor complex typically with IL2Rα (CD25) and IL2Rγ (CD132). This complex facilitates the response to IL-2 signaling which is essential for the proliferation and differentiation of immune cells. The receptor is also involved in the generation and maintenance of regulatory T cells important for immune tolerance.
Pathways
The soluble IL2 Receptor beta is involved in the IL-2 signaling pathway which controls T cell growth by altering gene transcription through the JAK-STAT pathway. IL2Rβ interacts with proteins like STAT5 and JAK1 which mediate signal transduction to achieve biological effects. This pathway is critical for immune responses and lymphocyte development where IL2 and similar cytokines regulate immune cell functions.
The role of soluble IL2 Receptor beta is significant in conditions like leukemia and autoimmune diseases. Elevated levels of soluble IL2Rβ often associate with hematological malignancies indicating tumor burden and disease activity in leukemia. Furthermore it has connections to proteins such as TNF-alpha in autoimmune diseases where they might contribute to inflammation and immune dysregulation highlighting its potential as a therapeutic target or biomarker.