Abcam, ab316747, Anti-TopBP1 (phospho S1138) antibody [EPR27056-2] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal TopBP1 phospho S1138 antibody. Carrier free. Suitable for WB, Dot and reacts with Transfected cell lysate - Human, Human, Synthetic peptide - Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR27056-2,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:Dot, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab316747 is the carrirer-free version of
ab316746
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TopBP1 also known as Topoisomerase II Binding Protein 1 is an important regulator in DNA replication and repair processes. This protein has an approximate mass of 180 kDa. It is expressed in various tissues with significant levels in rapidly dividing cells. TopBP1 functions mechanically by interacting with components of the DNA damage response machinery. It binds to double-strand breaks and activates ATR (Ataxia Telangiectasia and Rad3-related protein) signaling which is essential for maintaining genome stability under stress conditions.
Biological function summary
The activities of TopBP1 involve coordination of replication checkpoints and safeguarding the integrity of the replication fork. TopBP1 operates as part of a larger protein complex that senses DNA damage and initiates repair processes. It facilitates the recruitment of other repair proteins to sites of damage ensuring that replication proceeds only when the DNA is adequately repaired. It functions closely with proteins like RAD9 RAD1 and HUS1 which sense DNA damage and engage repair pathways.
Pathways
TopBP1 plays an important role in the DNA damage response and cell cycle regulation pathways. The protein is primarily involved in the ATR-Chk1 pathway and plays a significant role in the replication stress response. Within these pathways TopBP1 associates with proteins like ATR and Chk1 to coordinate cell cycle arrest and DNA repair linking replication and repair functions to maintain cell stability. It also interacts with the MRN complex (MRE11 RAD50 and NBS1) which is critical for responding to double-strand breaks.
The malfunction of TopBP1 has implications for cancer development and progression. Deficiencies or mutations in TopBP1 may result in impaired DNA repair leading to genome instability a condition frequently observed in cancerous cells. TopBP1 interacts with other tumor suppressors such as p53 to regulate cell cycle checkpoints and prevent the accumulation of mutations. Aberrations in TopBP1 function can also contribute to the pathology of developmental disorders as its interactions with proteins involved in genome maintenance are important for normal cellular function.