Abcam, ab316763, Anti-SNRP70/U1-70K antibody [EPR28616-89] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal SNRP70/U1-70K antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR28616-89,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human, Mouse, Rat,
Applications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab316763 is the carrirer-free version of
ab316762
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SNRP70 also known as U1-70K is a protein component of the spliceosomal U1 small nuclear ribonucleoprotein (snRNP) complex. It has a molecular mass of approximately 70 kDa. This protein is mainly expressed in the nucleus of eukaryotic cells. SNRP70 binds to the U1 snRNA facilitating the early stages of pre-mRNA splicing. It stabilizes the binding of the U1 snRNP to the 5' splice site therefore playing a role in recognizing and defining correct splice sites necessary for mRNA maturation.
Biological function summary
This protein is integral to the spliceosome a large multi-component complex responsible for removing introns from pre-mRNA. SNRP70 interacts with other core components of the U1 snRNP such as U1A and U1C proteins to assemble the complex. It ensures the accuracy of splice site recognition and contributes to the dynamic rearrangement of the spliceosome during splicing. These interactions importantly influence the regulation of gene expression by facilitating alternative splicing which allows a single gene to code for multiple proteins.
Pathways
SNRP70 is actively involved in the mRNA processing pathway a critical process in gene expression regulation. Its function directly links to the RNA splicing pathway ensuring the generation of mature mRNA from precursor transcripts. SNRP70's role in the spliceosomal complex places it in association with other spliceosome-related proteins such as SF3B1. This interconnectedness with SF3B1 supports the protein's engagement in ensuring accurate splicing and modulating gene expression through alternative splicing events.
Disruptions in SNRP70 function associate with certain autoimmune conditions and neurodegenerative diseases. For instance autoantibodies against SNRP70 are characteristic in patients with systemic lupus erythematosus (SLE) an autoimmune disorder. Also aberrant splicing events involving SNRP70 contribute to the pathology of certain neurodegenerative diseases such as spinal muscular atrophy. In these conditions the misregulation of splicing factors can exacerbate disease progression linking SNRP70 with other autoantigen proteins like Sm proteins in SLE.