Abcam, ab316877, Alexa Fluor® 555 Anti-EMR1/ADGRE1 (F4/80) antibody [EPR23225-94]

Size: 100µL
Rabbit Recombinant Monoclonal F4/80 antibody - conjugated to Alexa Fluor® 555. Suitable for ICC/IF, Flow Cyt and reacts with Transfected cell line - Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR23225-94,
Isotype:IgG,
Conjugation:Alexa Fluor® 555,
Excitation/Emission:Ex: 555nm, Em: 565nm,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IF, Flow CytSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:This antibody has not been tested in endogenous samples.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle, Store in the dark

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
EMR1 also known as ADGRE1 or EGF-like module-containing mucin-like hormone receptor-like 1 is a protein that belongs to the adhesion G protein-coupled receptor family. It has a predicted mass of approximately 147 kDa. This protein localizes primarily to the cell membrane and expresses in myeloid cells such as macrophages and dendritic cells. Its role involves cell signaling and interaction with other membrane proteins. EMR1 contains an extracellular domain with epidermal growth factor (EGF)-like modules which are critical for cell adhesion processes.
Biological function summary
The function of EMR1 plays an important role in the immune system especially in myeloid cell function. It does not exist alone but rather forms a part of the complex cell adhesion molecules contributing to cell-cell interactions and migrations. By doing so it supports immune surveillance and inflammation processes. It impacts the differentiation and survival of macrophages hence directly influencing host defense.
Pathways
EMR1's involvement extends to several cellular signal transduction pathways. Notably it plays a part in the immune response pathway and the GPCR signaling pathway where it interacts with related proteins such as CD11b and other integrins. These pathways ensure proper communication and activation of immune cells which is important for a coordinated immune response. Its interaction with other adhesion molecules suggests an integrated role in initiating signaling cascades that affect immune cell positioning and activation.
EMR1 shows a connection to disorders like inflammation and atherosclerosis. Its expression level often changes in chronic inflammatory conditions implicating it potentially in disease progression. The aberrant signaling of EMR1 can influence pathologies associated with dysregulated immune responses. Moreover in atherosclerosis EMR1's interaction would likely involve proteins like ICAM-1 and VCAM-1 further linking its activity to vascular inflammation and plaque formation.