Abcam, ab316889, Human SynCAM/CADM1 ELISA Kit

Size: 1 x 96Tests
Human SynCAM/CADM1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human SynCAM/CADM1 with a sensitivity of 14.525 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Serum, EDTA Plasma, Citrate plasma, Heparin Plasma,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 14.525 pg/mL,
Range:46.875 - 3000 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human SynCAM/CADM1 SimpleStep ELISA
kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of SynCAM/CADM1 protein in Serum, EDTA Plasma, Cit Plasma, Hep Plasma. Quantitate Human SynCAM/CADM1 with 14.525 pg/ml sensitivity.
SimpleStep ELISA
technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA
plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA
protocol summary in the image section for further details. Our SimpleStep ELISA
technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA
microplate (
ab203359
) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SynCAM also known as CADM1 (Cell Adhesion Molecule 1) is a protein that plays an important role in cell adhesion processes. It has an approximate mass of 105 kDa. SynCAM/CADM1 is expressed in various tissues including the brain where it supports synapse formation and maintenance. It is a member of the immunoglobulin superfamily and contains several I-set V-set and C2-set domains which contribute to its adhesion properties.
Biological function summary
SynCAM/CADM1 acts as a cell recognition molecule participating in the formation of synaptic connections in neural circuits. It is a component of a complex that regulates synaptic clustering and neural connectivity. In the nervous system SynCAM/CADM1 facilitates the initial axon-dendrite contact allowing efficient synaptic communication. Its expression and interaction affect the building of mature and functional synapses which are essential for cognitive processes.
Pathways
SynCAM/CADM1 integrates into synaptic development and cell signaling pathways. It interacts with scaffolding proteins like PSD-95 which stabilize the synaptic structure. The protein also participates in the Ras signaling pathway influencing cell growth and survival signals. Through its engagement in these pathways SynCAM/CADM1 plays a role in neural circuit plasticity and function maintaining proper communication between neurons.
SynCAM/CADM1 shows connections to neurological conditions such as schizophrenia and autism spectrum disorders. Its dysregulation is associated with abnormal neural connectivity leading to impaired cognitive function. SynCAM/CADM1's interaction with the L1CAM protein has been implicated in these disorders as both are involved in neural adhesion and signaling pathways that when altered can contribute to the development of such conditions.